Researchers are actively exploring the locally available fruits that may use for therapeutic remedies. Eleiodoxa conferta is an underutilized Malaysian fruit. This study was conducted to examine bioactive compounds and toxicity activities of different parts of E. conferta using 100% ethanol, 50% ethanol, and water for extraction. Results showed that Ethanol (50% v/v) was the best extraction solvent. The highest and lowest yield shown in the flesh extract and seed extract at 39.247% and 4.89%, respectively. The peel of E. conferta always showed higher Total Phenolic Content (TPC) and Total Flavonoid Content (TFC) as compared to other parts of the plant. Phytochemical compounds such as flavonoids, phenol, tannin, to name a few, were abundantly present in most extractions. The toxicity screening revealed that the toxicity level was high in the seed part of E. conferta while low toxicity shown in the flesh of the fruit. Overall, this fruit is rich in bioactivities. Further studies can be done to uncover its true potential application, especially the application of those bioactive components as antimicrobial agents in preserving food.