This study is on assessing the knowledge, attitude and factors affecting team building activities amongst health workers in Nigeria. The objective of this study is to determine the equality on knowledge and attitude of team building activities between health workers of Anambra state and Delta State. The source of data for this study was questionnaire, administered randomly to a sample of 200 workers at Anambra State and a sample of 305 workers at the Delta State. The test statistic used was the permutation method for Hotelling T-squared. The result of the analysis showed that there exist a significant difference on knowledge, attitude and factors affecting team building in Anambra State and Delta State with a test statistic value of 8073.7 and a p-value of 0.00 for 10, 000 permutations. This result indicate that the obtained significant value of 0.00 fall on the rejection region of the hypothesis assuming a significance level of 5% (α = 0.05) and implies that there is significant difference on the knowledge, attitude and factors affecting team building in the two States. Suggestion for the management of health in Anambra State to encourage the practice of team building by either sponsoring training of staff on team building or organizing seminars that will enhance the practice of team building was made since as argued in the present study that the benefit of team building in any organization is to achieves cohesiveness, improve the team attitude, effectiveness and enhances productivity. Studies on assessing the knowledge, attitude and factors affecting team building in other professional sector such as education, finance and environment in Nigeria is seen as an area for future research.