This study was aimed to prepare dental impression material from Sargassum duplicatum taken from Binuangeun, Banten. The setting time was tested according to ANSI/ADA Specification for Alginate Impression Materials 1983. After extraction, obtained the alginate powder then mixed its water/powder ratio from the combination of alginate composition and tested final setting time. Variations of the formulations used different calcium sulfate (0,1 g; 0,2 g; and 0,3 g). Product quality parameters are lumps, solubility, consistency, flow, tearing, setting time. The alginate yield obtained from Sargassum duplicatum by 31 % with characteristic gel strength by 816 (gr/cm 2 ); viscosity 238 mPas (2%; room temperature). The highest ratio is the best ratio by adding calcium sulfate powder of 0.1 gram with a setting time of 3 minutes.
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