The progressive freeze concentration of sucrose solutions was tested. The effect of the initial concentration of the solution (C 0), the temperature of the refrigerant (T) and the stirring speed (ω) on the final concentration of the solution was determined. The effects were significant on the freeze concentration, for both individual and combined effects. The maximum concentration achieved in the progressive freeze concentration was 53º Brix, when the initial concentration was 35º Brix, at a speed of 800 rpm and a temperature of refrigerant of-20°C. The best values of the concentration index are obtained at low concentrations, high stirring speed and low temperature. The average distribution coefficient increased with the initial concentration of the solution. The average yield parameter at different initial concentrations is around 0.6 kg ice•kg sol-1 •h-1 .

POSTERSMethod: Isolates of Staphylococcus aureus and Pseudomonas aeruginosa were cultured and divided into 3 groups. Group 1 was the control group, Group 2 was treated with saline delivered with hydrodynamic force, and Group 3 was treated with both hydrodynamic force and a near infrared diode laser.Results: For both the bacterial species, the use of laser augmented the reduction of Colony Forming Units obtained with hydrodynamic shearing, with an additional reduction in CFUs counts ranging from 0.94-2.18 logs and 0.68-1.15 logs for P Aeroginosa and S Aureus respectively. The overall log reduction obtained by combined modality treatment ranged from 4.57-5.06 for P Aeroginosa and 3.55-3.67 for S Aureus; both of these were statistically significant (P < .005).Conclusion: A combination of hydrodynamic therapy and laser produces superior results in disrupting biofilm in in vitro models, and further studies of this combined modality treatment are warranted in living tissues and animal models. Rhinology/AllergyObjective: Evaluate if the clinical measures SNOT-22 and UPSIT are efficient in monitoring treatment in Brazilian patients with CRS.Method: A total of 16 patients with CRS were treated with topical mometasone (200 microgram BID) for 2 months. SNOT-22, sense of smell score in SNOT-22, UPSIT, Lund-Mackay (for CT) and Lund (for endoscopy) scores were evaluated before and after treatment. Multi-variable correlation tests were used to compare the differences between scales.Results: Improvement in UPSIT scores correlated negatively to the sense of smell score in SNOT-22, (R2: 0.50; P = .0367), demonstrating to be a good tool to quantify smell problems. On the contrary, UPSIT did not correlate with CT and endoscopy scores. Improvement in SNOT-22 scores correlated significantly to improvement in endoscopy scores (R2: 0.49; P = .0297), but not to CT and UPSIT scores.Conclusion: UPSIT is a valuable tool to measure smell changes and monitor the effect of clinical treatment in patients with CRS. SNOT-22 correlated positively to intranasal polyp formation rather than sinusal opacity on CT scans.