In this study, the impact of treated sewage water was tested using 6 isolates of Anabaena oryzae by determination of Chlorophyll-a, Carotenoids and dry weight. In this study, treated sewage water was utilized to grow Anabaena oryzae for 6 weeks compared with Anabaena oryzae grow in BG-11 Medium as a control. The results showed the sewage water effects and correlation on Chlorophyll-a, Carotenoids contents and dry weight, As a result adaptations, resistance to organic pollutants and nutrients showed that Anabaena oryzae able to survive because these isolates modification with growth condition. The results by Gel electrophoresis showed genetic variations among Anabaena oryzae isolates DNA by using OPC-01 random primer.