The effect of heterosis on callus induction, callus mass and number of regenerated plants from mature embryo cultures of winter durum wheat (Triticum durum Desf.) hybrids was studied. A total of 14 F 1 hybrids and their parents were used for mature embryo culture. The statistical analysis of the results revealed that positive heterosis in callus mass was determined in only one F 1 hybrid and in number of regenerated plants in two F 1 hybrids. Plants regenerated in vitro were successfully established in soil. Hybrid genotypes may be used to obtain callus and regenerated plants with vigour comparable to their parents.