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Energy independence of the country can be solved in several ways. One of them is the creation of energy plantations of woody plants. The area increase of such plantations is limited by the insufficient amount of high-quality planting material. Hybrid plants of the genus Populus are of considerable interest when creating plantations. Given that not all hybrids are successfully propagated by conventional vegetative methods, the use of biotechnological methods, namely microclonal propagation, solves the mentioned problem. This method allows obtaining a large amount of highquality, uniform planting material. The purpose of this study was to develop the technology of microclonal propagation of black poplar hybrids ‘San Giorgio’ and ‘Ghoy’. The study used generally accepted biotechnological methods in the author’s modification at all stages of obtaining planting material of regenerating plants of poplar hybrids. Studies have established a positive effect when obtaining an aseptic culture of sterilising substances 0.1% AgNO3, 2.5 and 1.25% NaClO, 0.05% sodium merthiolate and 0.1% HgCl2. The largest number of aseptic viable explants was obtained by cultures using a 0.05% solution of sodium merthiolate after exposure for 5 and 10 minutes. When rooting microshoots on a hormone-free nutrient medium ½ MS and with the addition of 0.1-1.0 mg/l IBA, it was established that the best parameters for rooting microshoots are on a hormone-free nutrient medium ½ MS. Adaptation was carried out for 4 weeks in a greenhouse on a substrate that included components of peat:sand:perlite in a ratio of 1:1:1. The viability of regenerating plants of black poplar hybrids ‘San Giorgio’ and ‘Ghoy’ was more than 91%. The conducted research and the obtained results will be useful for producers of planting material and the scientific environment, which develop renewable energy sources through the creation of energy plantations of fast-growing woody plants

та вивчення усіх чинників, з урахуванням глобального потепління клімату, дало змогу запропонувати заходи з підвищення біологічної стійкості майбутніх лісів на етапі їх відтворення. Розуміння і використання викладених вище положень щодо етіології та особливостей патогенезу масового всихання дерев і насаджень лісотвірних порід дають можливість превентивно застосовувати науково обґрунтовані лісогосподарські заходи, які унеможливлюють вищеперераховані обставини ослаблення та ризику, і, тим самим, запобігти прояву шкодочинної дії першопричин, що допоможе мінімізувати негативні наслідки патогенезу та зменшити можливі ресурсні, екологічні й соціальні втрати.

Modern technologies make it possible to obtain nanoparticles of biogenic metals for use as an additional source of micronutrient for plants. However, the complexity of mass application of nanosized metal particles and their oxides is due to the significant differences in physicochemical properties of nanocrystalline structures which are dependent on production technology, nanoparticle size, surface charge (-potential), and stabilization methods. The biocompatibility and nature of nanoparticles has an impact on living organisms. Regarding the effectiveness and feasibility of using cerium dioxide nanoparticles in crop practice, there is no definitive conclusion. Due to difficulty in the preparation of planting material for seedlings of conifers, the study of the effect of nanocrystalline cerium dioxide on plants is not well researched. The aim of our research was to study the effect of nanocrystalline cerium dioxide solution on the germination of spruce seeds and then to evaluate its effect on the synthesis of phenols as components of the antioxidant system within seedlings. The research used methods for determining the germination energy and seed similarities. Other methods used in this research were determining the content of phenolic compounds, flavonoids, and phenolic antioxidants. The results showed that nanocrystalline cerium dioxide in a concentration of solution from 0.1 to 1.0 mg/mL stimulates the germination of spruce seeds. Under the influence of nanoparticles at a concentration of 0.1 mg/L in the tissues of spruce seedlings increases the content of phenolic compounds. The increase in antioxidant activity of phenols in seedling tissues while decreasing their total amount at a concentration of nanocrystalline cerium dioxide from 0.5 to 1.0 mg/L occurs when increasing the total pool of flavonoids, which are determined by high antioxidant activity. Nanocrystalline cerium dioxide is a promising material for stimulating germination energy and on the overall germination of spruce seeds.