No abstract

Ex-situ bioremediation of leached cynamonic forest soil at initial diesel oil contamination of 6,000 mg kg(-1), 4,000 mg kg(-1) and 2,000 mg kg(-1) was investigated after biostimulation with inorganic fertilizers. It was found that the added nutrients had no effect on the decontamination of polluted soils. A precise and reliable approach for evaluation of the biodegradation process is proposed. It comprises application of sensitive and easily accessible diagnostic parameters and relations, calculated on the basis of n-alkanes and isoprenoids--pristane (2.6.10.14-tetramethylpentadecane, i-C19H40) and phytane (2.6.10.14-tetramethylhexadecane, i-C20H42) distribution.

Screening by gas chromatography with flame ionization detection and gas chromatography-mass spectrometry has been carried out on sludge extracts of wastewater treatment basins. Soxhlet extraction with trichlorotrifluoroethane was applied. The yields for petroleum hydrocarbons and PAH recovery were high, usually in excess of 90%. The proposed investigations permit a quick assessment of petroleum pollutants in the environment.

Two approaches are proposed for the identification of a contaminant caused by the spilling of oil or oil products in water. A capillary gas chromatography (CGC)-mass spectrometry (MS) method for oil spill identification is applied. The presented approaches describe the use of MS data of 18 selective ions of spilled product and the probable pollutant. The spill identification is accomplished on the bases of a quantitative comparison between the ion chromatograms of the samples taken from the probable pollutant and from the spill itself. The other approach is made by chemometric treatment of complete CGC-MS data.

No abstract