The purpose of the study was to examine the supplementation effects of glucomannan extracted from a porang tuber (GEPT) on body resistance and growth performance of broiler chickens. A total number of 160 one-day-old broiler chickens with the average body weight of 42.39±0.58 g was kept for 35 days. The study was arranged in a completely randomized design with 5 treatments and 4 replications (8 birds each). The dietary treatments were T0= basal ration, T1= basal ration with the addition of 0.05% GEPT, T2= basal ration with the addition of 0.1% GEPT, T3= basal ration with the addition of 0.15% GEPT, and T4= basal ration with the addition of 0.2% GEPT. Parameters measured were the population of lactic acid bacteria (LAB), Coliform count, relative weight of lymphoid organs (bursa fabricius and spleen), heterophils-lymphocytes (H/L) ratio, body weight gain, feed consumption, feed conversion ratio, and mortality. Data were subjected to analysis of variance (ANOVA) and continued with Duncan Multiple Range Test (DMRT) at 5% probability. The results showed that supplementation of the diets with GEPT significantly increased (P<0.05) LAB population, decreased (P<0.05) Coliform count in the jejunum and ileum, and H/L ratio, but did not affect the relative weight of lymphoid organs, and growth performance. In conclusion, diet added with 0.1% GEPT improved the balance of intestinal microflora and increased body resistance, without any negative effects on the lymphoid organs and growth performance of broiler chickens.

No abstract

Glukomanan porang tersusun dari monomer D-glukosa dan D-manosa dengan ikatan β-1,4. Ayam tidak memiliki enzim yang dapat mencerna ikatan β sehinggaglukomanan porang berpotensi sebagai prebiotik. Penelitian ini bertujuan untukmengamati perubahan konsentrasi short chain fatty acids (SCFA) dan potential Hydrogen (pH) dalam jejunum ayam broiler akibat suplementasi glukomanan porang (GP). Penelitian menggunakan 160 ekor ayam broiler umur 1 hari strain New Lohmann dengan rerata bobot badan 42,39 ± 0,58 gram yang dipelihara selama 35 hari. Percobaan menggunakan rancangan acak lengkap dengan 5 perlakuan dan 4 ulangan @ 8 ekor. Perlakuan berupa T0: ransum basal, T1: ransum basal + 0,05% GP, T2: ransum basal + 0,1% GP, T3: ransum basal + 0,15% GP, dan T4: ransum basal + 0,2% GP. Parameter yang diukur meliputi konsentrasi SCFA antara lain asam asetat, propionat, butirat, dan total SCFA serta pH jejunum. Data dianalisis menggunakan analisis ragam (ANOVA) dan dilanjutkan dengan uji Duncan (DMRT) pada taraf nyata 5%. Hasil menunjukkan bahwa suplementasi glukomanan porang dalam ransum dapat meningkatkan konsentrasi total SCFA tetapi tidak menyebabkan perubahan pH jejunum ayam broiler.

Glucomannan is consisted of D-glucose and D-mannose with β-1.4 linkages. Poultry had no enzyme to digest β linkage so that porang (Amorphophallus onchophyllus) glucomannan could be a prebiotic candidate. The study purposed to determine the effect of porang glucomannan on caecum bacterial composition (similarity, diversity, grouping of bacteria) using Terminal Restriction Fragment Length Polymorphisms method and performance (body weight gain, feed intake, feed conversion ratio) of broiler chickens. One hundred and eighty unsexed day old chicks (DOC) were divided into 6 treat-ments and 3 replicates (10 birds each) using completely randomized design (CRD). The treatments were P0 (basal feed), P1 (basal feed + 0.05% porang glucomannan), P2 (basal feed + 0.1% porang glu-comannan), P3 (basal feed + 0.15% porang glucomannan), KJ (basal feed + 0.1% commercial konjac glucomannan), and KM (commercial feed). The results showed that caecal bacterial composition of porang glucomannan treatment had similarity with konjac glucomannan and basal feed. Diversity index and species number of porang glucomannan treatment was higher than konjac glucomannan. Gluco-mannan prebiotics had higher Lactobacillus while the lowest Clostridium was obtained in 0.1% porang glucomannan treatment. The inclusion of glucomannan prebiotics gave no negative effect on the per-formance of broilers.