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The location of glyoxylate-complex molecules has been investigated in several areas of the rat cerebral cortex using the immunohistochemical peroxidase-antiperoxidase (PAP) method. Antibodies against glyoxylate-complex molecules have been developed in the rabbit after immunization with a glyoxylate-bovine serum albumin conjugate. Observations carried out with the light microscope demonstrated positive immunostaining at the membrane level of scattered neurons located in all cortical areas, mainly in cortical layer IV. The striate areas (17, 18, 18a) had both the greatest number of immunopositive neurons and the most intense ones. At the electron microscopic level, it was observed that in the striate areas an immunopositive reaction was located mainly in the periphery of synaptic vesicles of some nerve endings, and in both pre- and postsynaptic membranes of these synaptic structures. The presence of glyoxylic acid and glyoxylate-complex molecules in such areas leads us to suggest that these substances could play an important role in selected synaptic contacts in which some pyramidal and non-pyramidal neurons are involved.

No data about the nature or function of the first major Anisakis simplex allergen, named Ani s 1, is available. The aim of this study was to investigate the ultrastructural localization of this protein, to obtain further information about it. Ani s 1 was detected in secretory granules of the excretory gland and occasionally lining the main excretory canal. Our results suggest that Ani s 1 could be a secretory product and could have an enzymatic function related to mechanisms of infection. Electron microscopy immunohistochemistryFresh A. simplex larvae were fixed with 4% paraformaldehyde and 0.5% glutaraldehyde in 0.1 M PBS (pH 7.2) for 24 h at 4°C, dehydrated with an ethanol series and embedded in LR White resin Parasitol Res (2003) 89: 379-380

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