:"Fatsiflogin" is an original, nonsteroidal para-medical preparation from the leaves of Fatsia japonica with anti-rheumatic, anti-inflammatory and analgesic activities. Fatsioside D (tetraosid of hederagenin) as one of the major compound of "Fatsiflogin", was selected as a chemical markerfor the quantitative validation. The objective of this study is to develop a simple and reliable HPLC method for the determination of Fatsiosid D for quality control studies of "Fatsiflogin". FatsiosideD was purified by preparative HPLC and the structure of the compound was determined by NMR. HPLC separation was performed on an intertsil prep-ODS C18 column, with a solvent system water-acetonitrile. The UV detection is performed at 205 nm. The proposed HPLC method is linear in the range studied (r 2 > 0.999) for all the analytes. The method is precise with intra-and inter-day variations of less than 3.34%. The mean recoveries of the analytes range from 99.68 to 100.29%. The method is successfully applied to the quantification of Fatsioside D. The results indicated that developed HPLC method could be used for the quality control of "Fatsiflogin".