We demonstrate the first realization of immuno-Raman microspectroscopy, a novel methodology in immunohistochemistry. The in situ detection of antigens in tissue specimens is achieved by surfaceenhanced Raman scattering (SERS) from aromatic Raman labels covalently linked to the corresponding antibody. As an example of applications of this new Raman technique, we present the localization of prostate-specific antigen (PSA) in epithelial tissue from patients with prostate carcinoma (PCA).

We present a quantitative investigation by linear Raman spectroscopy of a methane/air premixed flame from a commercial burner. Rotational temperatures and absolute densities of the major species, N 2 , O 2 , CH 4 , CO 2 , and H 2 O have been measured in a grid of points across the flame section. Axial and radial profiles of temperature and densities are reported, and mass balances along the flame axis are discussed. We show the capabilities of this technique for combustion studies by using currently available standard detectors and spectrometers.