Oral potentially malignant lesion(OPL), Rose Bengal stain (RB).Background: Oral cancer is a major public health problem in India usually preceded by precancerous lesions. Among all diagnostic aids, vital staining is simple, inexpensive and sensitive tool for identifying epithelial dysplasia and can be used for screening. Hence in the present study an attempt was made to evaluate efficacy of RB stain in early detection of oral potentially malignant among tobacco chewers and correlate the intensity of Rose Bengal stain with the degree of dysplasia. Objectives: To determine histopathological changes in biopsy specimens taken from tobacco chewers with positive and negative stain. To correlate intensity of the stain clinically with histopathological features taken from tobacco chewers. Methodology: The study comprised of 105 subjects with tobacco chewing habit for more than a year. RB stain was applied in the area of tobacco placement. Based on areas of highest uptake of stain, incisional biopsy was done. The intensity of uptake of stain was compared with the histological findings. Statistical analysis: One-way analysis of variance (ANOVA) test was applied. Results: RB stain was taken up in all tobacco chewers with or without lesions. Sensitivity was 93.33% Conclusion: RB stain is simple, cost-effective, non-allergic and is not taken up by inflammatory cells unlike Toluidine Blue stain. Sensitivity of RB staining method was found to be higher when compared with Toluidine Blue staining. Thus RB stain can be used as a diagnostic test in screening of potentially malignant lesions.

Age estimation, modified Kvaal"s formula, coronal pulp cavity index(CPCI), maxillary second premolar, periapical digital radiographs, paralleling technique.Background: Age determination has become increasingly important in forensic science not only for the identification of corpses, but also to clarify legal queries in delineating juvenile and young adults in spheres pertaining to employment, labour acts and criminal offences. It plays a crucial role in forensic medicine, especially in connection with crimes and accidents. Aim: To estimate the accuracy of age by using modified Kvaals formula and Coronal pulp cavity index. Objectives: 1. To assess the accuracy of modified Kvaal"s formula and Coronal pulp cavity index in estimating age among Indian population. 2. To compare the standard error of estimated age among both technique. 3. To establish the standard technique in assessing age among 5 groups. Methodology: 100 individuals were selected within age group of 20-70 years. Digital intraoral periapical radiographs was taken by using KODAK 8000 RVG machine with the exposure factors of 65 KVp and 8 mA for 0.2 s for either right or left side of maxillary second premolar by paralleling technique and maximum length of tooth, root and pulp were measured based on Modified KVAAL"s method and CPCI using the KODAK Dental Imaging software. Statistical analysis:The results obtained will be analyzed by using Descriptive analysis, Chi square test and ANOVA Results: Modified kvaals formula and CPCI can be used in estimation of age among Indians. To compare SEE between both techniques, Modified Kvaals method shows lesser error when compared with CPCI.