In spring-type oat ( Avena sativa L.), quantitative trait loci (QTLs) detected in adapted populations may have the greatest potential for improving germplasm via marker-assisted selection. An F(6) recombinant inbred (RI) population was developed from a cross between two Canadian spring oat varieties: 'Terra', a hulless line, and 'Marion', an elite covered-seeded line. A molecular linkage map was generated using 430 AFLP, RFLP, RAPD, SCAR, and phenotypic markers scored on 101 RI lines. This map was refined by selecting a robust set of 124 framework markers that mapped to 35 linkage groups and contained 35 unlinked loci. One hundred one lines grown in up to 13 field environments in Canada and the United States between 1992 and 1997 were evaluated for 16 agronomic, kernel, and chemical composition traits. QTLs were localized using three detection methods with an experiment-wide error rate of approximately 0.05 for each trait. In total, 34 main-effect QTLs affecting the following traits were identified: heading date, plant height, lodging, visual score, grain yield, kernel weight, milling yield, test weight, thin and plump kernels, groat beta-glucan concentration, oil concentration, and protein. Several of these correspond to QTLs in homologous or homoeologous regions reported in other oat QTL studies. Twenty-four QTL-by-environment interactions and three epistatic interactions were also detected. The locus controlling the covered/hulless character ( N1) affected most of the traits measured in this study. Additive QTL models with N1 as a covariate were superior to models based on separate covered and hulless sub-populations. This approach is recommended for other populations segregating for major genes. Marker-trait associations identified in this study have considerable potential for use in marker-assisted selection strategies to improve traits within spring oat breeding programs.
There has been considerable activity in breeding orchardgrass (Dactylis glomerata L.) cultivars in North America during the latter half of the 20th century, but little effort devoted to quantification of breeding progress. The objectives of this study were to quantify changes in mean cultivar performance for that time compared with the progress achieved from one cycle of half‐sib progeny selection within the USDA population of orchardgrass accessions. Forty‐two cultivars (32 North American cultivars and 10 European cultivars) were tested at three locations (Arlington, WI and Rock Springs, PA, and Ottawa, Ontario, Canada) in 1995 through 1997. Cultivars were grouped into three experiments by maturity class: early, medium, and late. North American cultivars averaged 3, 9, and 12% higher in forage yield than European cultivars for early, medium, and late maturity groups, respectively. Between 1955 and 1997, forage yield and ground cover of early‐maturity cultivars increased by 2.5 Mg ha−1 decade−1 and 4.0% decade−1, respectively. Forage nutritional value of medium‐maturity cultivars increased during that time, although this was probably not due to direct selection. Significant gains were made in forage yield and Drechslera spp. leafspot reaction of cultivars derived from two individual breeding programs, although the majority of orchardgrass cultivars lack improvements in forage traits.
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