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Bacteriostatic action of a biocidal agent results from the cumulative impact of different kinetics, including those of bacterial growth, mass transfer of the agent and its antibacterial action against the targeted bacteria. Current studies on bacteriostatic effects always directly consider the combination of these kinetics at given times, without discrimination between each other. This work introduces a novel approach, consisting of first studying independently, by the experiment and the model, the different kinetics involved, and then in coupling these kinetics to obtain a model that will be confronted with experimental data. An agar diffusion test with silver ions against Escherichia coli bacteria was implemented herein to assess the relevance of this approach. This work achieved to characterize the different kinetics and to propose a dynamic model combining them, which fits the experimental data with a silver diffusivity in the biofilm fixed to 7.0 ± 0.1 × 10−12 m2 s−1. This study also proves that the diffusive phenomenon was limiting the bacteriostatic action of silver ions over the test duration.

Artícu lo ci entíf ico Molecular characterization of Bradyrhizobium japonicum J-01, J-96 and J-98, rep-PCR using protocolsCaracterización molecular de las cepas de Bradyrhizobium japonicum J-01, J-96 y J-98, mediante protocolos rep-PCR