Background and Aim: Fasciolosis due to Fasciola gigantica is endemic to tropical countries and Fasciola hepatica in temperate climates, highly detrimental to livestock and known as foodborne zoonotic diseases. The strategic control of the disease is mainly the use of chemical anthelmintic. This study aimed to evaluate the anthelmintic properties of Artemisia vulgaris extract on the ova and adult stages of F. gigantica. Materials and Methods: Samples were collected from the Ampel Abbatoir, Boyolali District, Central Java, Indonesia. The ova from 20-gallbladders of cattle which were naturally infected with F. gigantica and 270 living F. gigantica worms were used in this study. The ovicidal assay was performed by incubating the ova with A. vulgaris in different concentrations, that is, 5%, 2.5%, and 1.25% for 5, 9, 11, 14, and 16 days. The efficacies were evaluated by quantification of ova degeneration during developmental stages in different time points and egg-hatch assay. The flukicidal effects were observed by mortality assay in 5, 10, 20, 40, 80, 160, 320, and 640 min incubations followed by scanning electron microscopy for surface morphology and histology of the fluke’s transversal sections. Results: The concentration of 5% A. vulgaris showed the strongest ovicidal activities. The percentage of hatching ova on day 16 at concentrations of 5%, 2.5%, and 1.25% were 3.33%, 6.67%, and 16.67%. These ova hatch assay showed a significant reduction (p < 0.001) compared to untreated control. The flukicidal effect was significant (p < 0.001) at a concentration of 20%, with a mortality rate reaching 66.67% in the 40 min of incubation time. The surface properties of the adult worms, including the spine, tegument, acetabulum, intestine, and vitelline follicles, were disintegrated. Conclusion: The results showed that A. vulgaris has the potential ovicidal and flukicidal properties to F. gigantica. The active compounds remained necessary to be elucidated further and its modes of action would be interesting to be predicted by molecular docking modeling. Keywords: anthelmintic, Artemisia vulgaris, Fasciola gigantica, flukicidal, ovicidal.

The use of plants as natural anthelmintic is quite promising for parasite control. In this report, we evaluate the activity of Etlingera elatior to Fasciola gigantica eggs in vitro. The eggs were collected from naturally-infected bovine in the Ampel Abbatoir, Boyolali, Central Java. The eggs were exposed to E. elatior ethanolic extract in different concentrations i.e., 5%, 2.5% and 1.25%. Fluconix 340 in the equal concentrations was positive control of anthelmintic and distilled water served as negative treatment. The eggs were placed in the dark until 9 days and observed under the light microscope on days 5 and 9. The results showed that E. elatior in the concentration of 5%, 2.5% and 1.25% significantly inhibited the initial development of F. gigantica compared to the negative control (water) on days 5 and 9 treatments (P<0.05). The E. elatior effectively inhibits morulation and larva formation. Fluconix 340 5%, 2.5% and 1.25% were possessing higher ovicidal activities compared to E. elatior since day 5 of treatment. E. elatior showed effective inhibition to the initial development of F. gigantica egg in vitro.