With our adaptations it is possible to discriminate tandem conjugates of Cy5, Cy5.5, and Cy7 for eight-color immunophenotyping. Using this method, novel rare subsets of NK and NKT cells that are CD4/CD8 double positive are reported for the first time.

No abstract

No abstract

No abstract

\s=b\Individual chemosensitivity assessment of solid tumors has been hindered by the lack of a reliable and rapid test system for predicting inhibition of tumor cell growth in vitro of all malignant cells and for distinguishing between inflammatory and tumor cells. Six long-term cultured and recloned squamous cell carcinoma lines of the head and neck were analyzed using a newly developed multiparametric flow cytometric technique. After incubation with cytotoxic drugs at different time intervals, each cell line showed its own chemosensitive profile. With this multiparametric staining technique, it is possible to monitor the in vitro response of tumor cells and to compare their quantitative behavior with that of nonmalignant inflammatory cells in solid tumors of the head and neck.