Covalent coupling of bovine rhodopsin to CPG-thiol glass was used for separation of CNBr peptides. It is shown that cysteine residues 322 and 323 in the C-terminal cytoplasmic fragment of rhodopsin are modified with palmitic acid.
Addition of H 2 O 2 in sub-millimolar concentrations to 1-5 mM Na/K-bicarbonate solutions initiates in them a process accompanied with low-level photon emission amplified with luminol. Photon emission lasts without decay for many months in samples isolated from air and ambient light. Photon emission intensity reveals monthly and circadian rhythms. Amplitude of photon emission intensity from test-tubes filled with active solutions increases two-to threefold on periods coinciding with the eclipses of the moon and the sun. Preparations of water-soluble fullerenes in dilutions equivalent to their concentrations 10 -13 , 10 -15 and 10 -19 -10 -20 M increased photon emission two-to threefold while intermediate dilutions affected it much weaker. We suggest that addition of H 2 O 2 to aqueous bicarbonate solutions initiates in them cyclic chain reactions in which water is oxidized with oxygen. Bicarbonate may stabilize these cyclic reactions due to its ability to participate in free radical reactions. Extremely long-lasting photon emission (high density electromagnetic energy) from activated bicarbonate solutions indicates that they reside in stable non-equilibrium (excited) state supported presumably by continuous efflux of low density (electromagnetic) energy from the environment. Such systems may represent a model of Confined Ontic Open Systems able to transform low grade energy into high grade energy. It is notable that bicarbonate is the necessary constituent of cytoplasm of aerobic cells and of important biological liquids, in particular of blood plasma. Normal and healing drinking waters also usually represent bicarbonate solutions.
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