This experiment was designed to evaluate the effects of lipid source and fatty acid (FA) profile on intake, performance, carcass characteristics, expression of enzymes, and sensorial analysis of B. indicus animals offered a high-concentrate diet. On day 0, 96 non-castrated animals were blocked by initial body weight (400 ± 19.3 kg), randomly allocated to 1 of 24 pens (4 animals/pen), and pens were randomly assigned to receive: 1) Control: basal diet composed of whole cottonseed and corn germ as lipid substrates (CONT; n = 6), 2) Calcium salts of fatty acids (CSFA) of soybean: CSFA of soybean oil as replacement for whole cottonseed and corn germ (CSSO; n = 6), 3) CSFA-Blend: CSFA of palm, cottonseed, and soybean oil as replacement for whole cottonseed and corn germ (CSVO; n = 6), and 4) Mix: basal diet containing whole cottonseed, corn germ, and CSVO (MIXT; n = 6). Experiment lasted 108 d and performance, ultrasound measurements, as well as carcass characteristics were evaluated. Additionally, meat FA profile, expression of enzymes involved in lipid metabolism, and sensorial analysis were evaluated. No treatment effects were observed on performance variables, ultrasound, and carcass traits (P ≥ 0.22), whereas animals receiving CONT had a greater intake of C10:0, C16:0, C16:1 trans-9, C18:1 cis-9, C18:2, C18:3, total FA, monounsatured FA (MUFA), and polyunsaturated FA (PUFA) vs. CSSO and MIXT (P < 0.05). Conversely, intake ratios of saturated FA (SFA):MUFA and SFA:PUFA were all reduced for CONT vs. other treatments. Meat obtained from CONT animals had greater colorimetric (L*, a*, and b*) values vs. MIXT (P < 0.01). On meat FA profile, CONT increased C18:0 vs. supplementation with calcium salts (P < 0.02) and supplementation with CSSO yielded greater meat concentrations of C18:1 trans-10 and C18:2 CLA intermediates (P < 0.01). Expression of SREBP-1, SCD, and LPL was downregulated for CSSO (P < 0.05). For sensorial analysis, regular flavor was greater (P = 0.01) for CSSO vs. other treatments, but also greater aroma (P = 0.05) vs. CONT and CSVO. In summary, addition of different lipid sources with varying FA profile into high-concentrate diets did not affect performance and carcass characteristics of B. indicus animals, but supplementation with calcium salts of soybean oil inhibited the mRNA expression of enzymes involved in lipid metabolism, whereas flavour and aroma were positively impacted by this lipid source.

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The effects of substituting ~50% of the soybeans in the diet of finishing Nellore steers with either urea (U) and/or slow-release urea (SRU) on the steer performance and meat quality were assessed in two experiments. In the first experiment, 46 Nellore steers in a 104-day experiment (Exp. 1) were fed a Control diet with U or SRU or U+SRU. In Experiment 2 (Exp. 2), digestibility and microbial protein (MCP) synthesis were assessed in four steers by using a 4 × 4 Latin square design with 21-day periods. Four corn-based diets were used in both Exp. 1 and 2. (1) Control (CTL): 0% of non-protein nitrogen (NPN). (2) U: 1.66% of NPN. (3) SRU: 1.8% of NPN. (4) U+SRU: 1.72% of NPN. In Exp. 1, final bodyweight, average daily gain, dry matter intake, Gain to Feed ratio, carcass traits and steer meat quality were not influenced by the experimental diets. In Exp. 2, the apparent digestibility was similar for all diets, and the MCP synthesis was affected by dietary treatments (P = 0.065). The NPN treatments showed 25.5% more (P = 0.03) MCP efficiency (g microbial protein/kg of total digestible nutrient content consumed) than the CTL. We conclude that the partial replacement of SBM with U, SRU or U+SRU will provide similar animal performance without negatively impacting carcass and meat quality and improve the efficiency of microbial protein synthesis in Nellore cattle.