This experiment was designed to evaluate the effects of lipid source and fatty acid (FA) profile on intake, performance, carcass characteristics, expression of enzymes, and sensorial analysis of B. indicus animals offered a high-concentrate diet. On day 0, 96 non-castrated animals were blocked by initial body weight (400 ± 19.3 kg), randomly allocated to 1 of 24 pens (4 animals/pen), and pens were randomly assigned to receive: 1) Control: basal diet composed of whole cottonseed and corn germ as lipid substrates (CONT; n = 6), 2) Calcium salts of fatty acids (CSFA) of soybean: CSFA of soybean oil as replacement for whole cottonseed and corn germ (CSSO; n = 6), 3) CSFA-Blend: CSFA of palm, cottonseed, and soybean oil as replacement for whole cottonseed and corn germ (CSVO; n = 6), and 4) Mix: basal diet containing whole cottonseed, corn germ, and CSVO (MIXT; n = 6). Experiment lasted 108 d and performance, ultrasound measurements, as well as carcass characteristics were evaluated. Additionally, meat FA profile, expression of enzymes involved in lipid metabolism, and sensorial analysis were evaluated. No treatment effects were observed on performance variables, ultrasound, and carcass traits (P ≥ 0.22), whereas animals receiving CONT had a greater intake of C10:0, C16:0, C16:1 trans-9, C18:1 cis-9, C18:2, C18:3, total FA, monounsatured FA (MUFA), and polyunsaturated FA (PUFA) vs. CSSO and MIXT (P < 0.05). Conversely, intake ratios of saturated FA (SFA):MUFA and SFA:PUFA were all reduced for CONT vs. other treatments. Meat obtained from CONT animals had greater colorimetric (L*, a*, and b*) values vs. MIXT (P < 0.01). On meat FA profile, CONT increased C18:0 vs. supplementation with calcium salts (P < 0.02) and supplementation with CSSO yielded greater meat concentrations of C18:1 trans-10 and C18:2 CLA intermediates (P < 0.01). Expression of SREBP-1, SCD, and LPL was downregulated for CSSO (P < 0.05). For sensorial analysis, regular flavor was greater (P = 0.01) for CSSO vs. other treatments, but also greater aroma (P = 0.05) vs. CONT and CSVO. In summary, addition of different lipid sources with varying FA profile into high-concentrate diets did not affect performance and carcass characteristics of B. indicus animals, but supplementation with calcium salts of soybean oil inhibited the mRNA expression of enzymes involved in lipid metabolism, whereas flavour and aroma were positively impacted by this lipid source.

The objective was to evaluate the effects of 25-hydroxycholecalciferol (HyD®, DSM Produtos Nutricionais Brasil S.A.) supplementation in expression of genes related to anabolism and catabolism of feedlot cattle. A total of 120 Nellore bulls (IBW = 370±20 kg) were distributed in 24 fully roofed-pens with concrete floor (5 animals/pen) in a randomized complete block design (8 pens/treatment). The treatments were T1 (control): no supplementation of HyD®; T2: HyD® supplementation at 1 mg/animal/day; T3: HyD® supplementation at 3 mg/animal/day. Basal diet was formulated to meet requirements of finishing bulls, considering an ADG of 1.6 kg/day (Level 2 Nutrition System, Fox et al., 2004). Adaptation to diets followed a step-up scheme for 14 days, with concentrate inclusion being gradually increased from 76 to 91% DM. Finishing diets were offered from 15th to 100th day of feedlot period and animals fed twice a day (0800 and 1500), with bunks managed for a maximum of 5% orts. After slaughter, muscle samples were collected for quantitative evaluation of gene expression using RT-qPCR method, considering the following genes: SOD1 (antioxidant marker), IGF1, IGF2 and MTOR (anabolism), FOXO1, MURF1, Atrogin-1 and MSTN (catabolism). Statistical analysis was performed using PROC MIXED of SAS® and means compared by Tukey test at 5% probability. No significant differences among treatments were observed for SOD1, FOXO1, MURF1 and Atrogin-1 expression (P >0.05). However, tendencies (0.05 > P ≤0.10) could be observed for IGF1, IGF2, MTOR and MSTN expression in animals receiving HyD®, regardless of dosage. These results indicate a positive effect of HyD® on muscular anabolism and protein synthesis on feedlot finishing cattle. Moreover, the greater expression on MSTN suggest a higher protein turnover and muscular growth regulation. In conclusion, HyD® supplementation increased expression of genes correlated to muscular growth and protein synthesis, being a viable technology for beef cattle finished in feedlot systems.

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O objetivo do presente estudo foi avaliar o desempenho e características de carcaça de bovinos Nelore castrados e não castrados terminados em confinamento com dieta de alto concentrado. Trinta e seis bovinos machos da raça Nelore com idade de 24 meses, provenientes de recria em sistema de pastejo contínuo, foram divididos em dois grupos de 18 animais (castrados e não castrados) com peso vivo inicial (PVi) médio de 347,5 kg ± 21,61 kg. O delineamento experimental foi em blocos casualizados, divididos de acordo com o PVi para cada condição sexual avaliada, constituindo desta forma dois tratamentos experimentais: animais castrados e animais não castrados. Cada tratamento foi composto por seis baias (3 animais/baia), sendo estas consideradas as unidades experimentais do estudo. O período de confinamento foi de 112 dias, sendo o abate realizado no 113º dia. Foram avaliados a ingestão de matéria seca (IMS), o ganho de peso diário (GPD), conversão alimentar (CA) e eficiência alimentar (EA). Além disso, foram avaliados a deposição de tecido muscular e adiposo por meio de ultrassonografia. Após o abate dos animais foram determinados o peso de carcaça quente (PCQ), o peso de carcaça fria (PCF) e rendimento de carcaça (RC). Os dados foram analisados utilizando-se o programa PROC MIXED do SAS  e teste de Tukey (P ‰¤ 5%). Não houveram diferenças significativas para as variáveis PVi e IMS em porcentagem de PV.  Já as variáveis PVf, GPD, CA e EA apresentaram efeitos significativos, tendo o grupo de animais não castrados  melhor desempenho produtivo. Para as avaliações de características de carcaça foram observadas diferenças significativas para PCQ e PCF onde os animais do grupo não castrado também apresentaram melhores pesos de carcaça. Portanto, conclui-se que para sistemas de confinamento com dietas de alto teor de concentrado, a castração de bovinos Nelore não é recomendada, pois interferiu negativamente no desempenho produtivo das carcaças dos animais castrados.

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