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The Junin virus is the agent of hemorrhagic Argentine fever. It was isolated in 1958 by Parodi et al. (1) from a human being. It has been tentatively classified as a member of Arboviruses by its biological and epidemiological features.In order to confirm its classification it is necessary to know in more detail its properties, size and the chemical nature of its nucleic acid.Junin virus reproduces not only in new born mouse brain, but also in guinea pigs in which case it is possible to observe a viremia till the death of the animal. Therefore, we used infected guinea pig plasma for our studies. Materials and Methods Virus. Plasma from guinea pigs infected with Junin virus, strain XJwith several passages in guinea pigs and mice was used.The dilutions of the virus were made in steril isotonic phosphate buffer pH 7.4 with 2% of inactivated normal rabbit serum, 1000 U/ml. penicillin and 100 ug/ml, streptomycin, in every ease, if not otherwise stated. The infected plasma was prepared by inoculation of guinea pigs (200--250grs. weight) with a 10 -3 dilution of virus in a dose of 0.2 ml. by intramuscular route. Eleven days after the inoculation they were bled and the blood collected with heparin.

hypophysis of iodine deficient animals was suggested by histologicat examination of their hypophysis. These glands showed a decreased percentage of acidophils with an increased number of basophils, as is the case with thyroidectomized a n i m a f~.~~' l8 Since this hypophyseal picture in thyroidectomized animals is indicative of excessive release of thyrotropic hormone,l6+ l9, 2o it is likely that it bears the same significanw in animals lacking iodine. Goitrous thyroids would thus result from hypophyseal modifications. However, the possibility of a direct action of iodine on the thyroid is not excluded."The hyperplastic thyroids of rats given an iodinedeficient diet acquire an increased ability to fix iodine. The thyroid and hypophyseal changes in these goitrous animals indicate an excessive release of thyrotropic factor from their hypophysis.Coml.tlsion. 13480 Use of Hamster (Cricetus auratus) for Detection of Influenza Virus in Throat Washings." 13. M. TAYLOR AND A. S. PARODI. (Introduced by J. H. Bauer.) F r o m the Pirus Dqinrtment of the Instituto Bacteriolo'gico, Buenos Aires.One of us has reported' that the hamster (Cricetus auratus) gave a specific immune response following intranasal inoculation of throat-washings taken from persons acutely ill with influenza A. These observations have now been extended, not only with regard to the immune response of the hamster but also as to its use in adapting influenza A virus to mice.ExficYiwenfnZ Mcrterinl. The throat-washings used in these experiments were collected from persons presumably ill from influenza during the epidemic which occurred in the Argentine in July, 1940. The throat-washings were taken with 20 cc of an equal mixture of 17 Marine, D., Rosen, S. H., and Sparke, C., PROC. SOC. EXP. BIOL. AND Mm.91sSliarpless. G. R.

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