Aim: To analyze the most effective Novel semen extender for long time cryopreservation among Tris egg yolk extender and Soya milk extender. Materials and Methods: The sample size was calculated using clinCalc.com with an alpha error-threshold at 0.05, 95 % confidence interval, G power at 80 %, and enrolment ratio 1. The study was repeated 14 times (N=14) and was measured using statistical Independent Samples Test. The study contained a total sample size of 28 with 2 different groups. Tris egg yolk and soya milk based novel semen extenders were added to the collected semen samples and few drops of the sample was placed on a makler counting chamber which was used for analyzing the concentration, sperm motility and sperm count of the semen samples. Results: The Tris egg yolk based semen extenders were better than the soya milk based semen extenders. Estimated sperm motility and sperm count were found to be P>0.05, revealing statistical insignificance. Conclusion: The sperm parameters were preserved well in Tris egg yolk based semen extender when compared with soya milk based semen extender with respect to sperm count and sperm motility.