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This investigation was carried out during the two successive seasons of 2006 and 2007 at the Agricultural Experimental and Research Station, Faculty of Agriculture, Cairo University Giza, Egypt. to study the effect of IAA and BA on vegetative growth and yield of soybean plant. Three concentrations, 50,100 and 200 ppm. were used of each growth regulator. The results revealed that IAA or BA increased main stem length, number of branches and leaves per plant, dry weight of leaves, stems and pods per plant, number of mature pods and seeds per plant and seed yield per plant. Number of main stem internodes, total leaf area per plant and weight of 100 seeds were not affected by treatment.

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Direct shoot regeneration of two flax cultivars; Blanka (a fiber cultivar) and Giza 8 (a dual purpose cultivar) was conducted in vitro using different explants from the seedling (hypocotyl, cotyledon and root). Factors affecting as well as stages and capacity of direct shoot regeneration and anatomical features of in vitro induced shoots comparing with in vivo shoots were studied. The most remarkable outcomes proved that the used culture medium is the key factor that affecting the shoot regeneration when combined with any other factor (explant type, explant age and genotype). Hypocotyl explant proved to elevate shoot regeneration percentages that varied between 33 to 95%. Three days aged explant bear out the highest percentage of shoot regeneration as compared with explant aged 7 days. The most medium was MS basel medium supplemented with 0.25 mg/l NAA + 0.50 mg/l BA. Inductive flax cv. Blanka showed a higher shoot regeneration percentage as compared with the other genotype flax cv. Giza8. Bud formation was initiated on the surface of the flax seedling hypcotyl as small projection, this projection showed a heavy wrinkled and waved sculpture, later it developed into normal shoot bud with mersitematic dome. The anatomical features of in vivo and in vitro stems and leaves were inversely distinctive as the in vitro stems were wider and maintained thicker epidermal cells as well as thicker cortex and pith whereas having lesser amount of supportive and vascular tissues. Comparing with in vivo leaves, the in vitro leaves were thiner with poor differentiated epidermal, mesophyll and vascular tissues. The in vitro leaves showed bigger and denser stomata area in both leaf surfaces.

The objectives of this investigation were to find out the remarkable morphological, histological and chemical differences among some wild taxa belong to genus Vicia, i.e., V. narbonensis var. narbonensis , V. narbonensis var. salmonea and V. serratifolia and the cultivated V. faba var. minor.Morphological characters reveal that; V. narbonensis var. narbonensis and V. narbonensis var. salmonea are more or less similar on this respect but differs from V. faba var.minor or V. serratifolia. The latter is very distinguishable from both varieties of V. narbonensis. V. faba var.minor has a unique characters differ from the other taxa.Studying the primary structure of root, stem and leaflet represents the histological differences among the wild taxa. The structure of the tap root (7 days old) presents remarkable differences in the types of vascular cylinder, being a trieach protostele in both varieties of V. narbonensis , tetrarch protostele in V. faba var.minor and pentarch protostele in V. serratifolia.V. serratifolia has the thickest tap root with widest vascular cylinder, which occupies the pith area among the studied taxa. In addition, V. serratifolia has the largest diameters of xylem arms and number of xylem vessels, while phloem area do not differ in other taxa. The morphological results indicate also that the secondary roots are three in both varieties of V. narbonensis, four in V. faba var. minor and five in V. serratifolia.The structure of the major and minor vascular bundles in the second stem internode below the shoot apex show that V.faba var.minor exceed all those of the other wild taxa.The histological structure of the leaflet shows that V. serratifolia has a characteristic property showing the six vascular bundles of leaflet arranged in two rows in the mesophyll, while other wild taxa and V. faba var. minor have one bundle only.Protein and non-protein amino acids results show slightly the same morphological results of taxa under investigation.