The breeding of camels in the world is growing expansionary due to increasing in consumption rate of its meat, milk and wool. Besides, The rise of profitability of this industry and importing of camels into the country through the South East borders make it important to recognize infectious diseases in this species. Theileriosis a fatal protozoan infection with worldwide distribution into the domestic animals. It has two important species, Theileria camelensis, and Theileria Dromedarii. Because of low reliability in the current visual detection of this diseases we designed this study to investigate the prevalence of camels Theileria in Zabol and Zahedan district.Blood of 310 tick infected camels were collected from the jugular vein during July 2014 to July 2005. DNA was extracted from a whole blood sample, and PCR determined the presence of the parasite.The results showed that none of the blood contains the DNA of Theileri Camelensis and Theileria Dromedarii This result suggests that this population of camels in the southeast of country is clean from the Theileri Camelensis and Theileria Dromedarii.