Ten samples of mixed feeds and one of lucern (alfalfa) were analysed. They were supplied by proces-siJig plants from Santa Fe Province, Argentina, and designed for feeding rabbits. Several breeding farms had e/aimed that these lots were the possible cause of intoxication. Tlie degree and type of fungal contaminatipn and the presence of the following mycotoxins were ana~vsed: aflatoxins (.4FI), ochratoxin A, citrinin, penicillic acid, zearalenone (ZE4) , and trichothecenes T-2 toxin, diacetoxyscirpenol (DAS) and deoxynivalenol (DON). The total fungdl coun/ was carried out by the dilution plating method,on dichloran rose bengal chloramphenicol agar (DRBC). Isolation ofspecies lI'as pelformed by means of direct plating on moist chambers aswell as thefolloll'ing media: Aspergiltusflavus-parasiticus agar (.4 FPA), chlorO/l/phenicol-potato-dextrose agar (PDAC) and dichloran18%g~vcerol agar (DG18). Detection and quantiflcation of mycotoxins were pelformed by means of thin layer chromatography (TLL). lt is no/ possible to assure tha! the intoxication cases observed by the breeders were caused by mycoloxins. However, the aflatoxins present in 7/ 11 sO/l/pIes and the high levels found in t1l'O of lhem (200 and 300 J1g/kg) could have been the reason.
Se estudió la influencia del medio de cultivo, la cepa, el tiempo y la temperatura sobre la biosintesis de tricotecenos en cinco cepas de Fusarlum toxicogénicas. Se analizó también el efecto combinado de dichas variables. Para la evaluación del grado de toxicidad se utilizó el micrométodo biológico basado en la inhibición que ejercen los tricotecenos sobre la germinación de semillas de Lepidium satlvum. (5).Analizando el efecto de las variables por separado, se constató que existe un 99% de probabilidad (<= 0.01) de que el medio de cultivo, la cepa y la temperatura incidan en la biosíntesis de tricotecenos; en tanto que para el tiempo, y dentro del rango estudiado, la probabilidad es del 95% (<= 0.05). Encuanto a la illteracción de las variables, existe un 99% de probabilidad (<= 0.01) de que el efecto combinado de medio de cultivo-tiempo-temperatura y medio de cultivo-cepa-temperatura incidan en el grado de toxicidad.
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