Background: Pulp capping should always be considered as the primary treatment of choice for teeth without irreversible pulpitis in lesions approaching dental pulp. The predictability of vital pulp therapy has improved with the introduction of newer bioceramic materials. Aim: The aim of this study was to evaluate the clinical success of Biodentine, calcium hydroxide (CH), and mineral trioxide aggregate (MTA) as pulp capping materials for indirect pulp capping in carious permanent teeth. Materials and Methods: Indirect pulp capping was done for 36 molars of 36 patients with deep caries lesions. They were randomly divided into three groups: Biodentine group (12 teeth), MTA group (12 teeth), and CH group (12 teeth). Patients were recalled at 1, 3, and 6 months to evaluate the clinical success of the treatment outcome. Statistical Analysis: All statistical analysis was performed using SPSS software version 21.0. Pearson's Chi-square test was used to compare the success and failure rates between Biodentine, MTA, and Ca(OH) 2 at three different time intervals (30, 90, and 180 days) and also the overall success and failure rates between Biodentine, MTA, and Ca(OH) 2 irrespective of the time intervals. P < 0.05 was considered statistically significant. Results: In a statistical trial/study, the pulp capping materials gave different success rates, 91.67% success in the Biodentine group, 83.33% success in the MTA group, and 58.33% success in the CH group. The results were not statistically significant. Conclusion: Indirect pulp capping with calcium silicate materials provided better results compared to that of calcium hydroxide.

Background: Nowadays, emergence of drug resistance might happen in the world. Hence, invention of new dental material had started by researchers for the same. The present study evaluated the antimicrobial property of green synthesized nanosilver particles against dental cariogenic microorganisms such as Streptococcus mutans and Lactobacillus acidophilus. Materials and Methods: An in vitro study had been designed to meet the objectives. Galla Chinensis ellagic acid powder synthesized nanosilver particles (GCAgNPs) synthesized nanosilver particles were used in this study. The cariogenic bacteria S. mutans (ATCC 25175) and L. acidophilus (ATCC 4356) were used in this study. The antimicrobial activity was detected at different concentrations (1000 μg/ml, 500 μg/ml, 250 μg/ml, 125 μg/ml, and 62.5 μg/ml) by means of qualitative and quantitative methods. Results: The results show a statistically significant difference between all the concentration (1000 μg/ml, 500 μg/ml, 250 μg/ml, 125 μg/ml, 62.5 μg/ml) in Galla Chinensis synthesized silver nanoparticles (GCAgNPs) in S. mutans and L. acdiophilus. Intergroup comparison of GCAgNPs shows a statistically significant difference among all the concentrations against S. mutans and L. acidophilus. Conclusion: GCAgNPs show antimicrobial and antibiofilm activity against S. mutans and L. acidophilus microorganisms.

Background: Dental caries is one of the most common chronic diseases, and it is caused by the acid fermentation of bacteria that have become attached to the teeth. Streptococcus mutans (S. mutans) and Lactobacillus acidophilus (L. acidophilus) anchor surface proteins to the cell wall and form a biofilm to aid adhesion to the tooth surface. Some natural plant products, particularly several flavonoids, are effective inhibitors. However, given the scarcity of inhibitors and the emergence of drug resistance, the development of new inhibitors is critical. The high-throughput virtual screening approach was used in this study to identify new potential inhibitor of against S. mutans and L. acidophilus by using ligand (Ellagic acid). Aim: To evaluate the drug interaction ligand (Ellagic acid) and protein [A3VP1 of AgI/II] of Streptococcus mutans (PDB ID: 3IPK), glucan-1,6 - alpha-glucosidase from Lactobacillus acidophilus NCFM (PDB ID: 4AIE). Materials and Methodology: The pdb format of two selected proteins was retrieved from the RCSB protein database. Then inhibitors were docked with protein (A3VP1 of AgI/II) and glucan-1,6-alpha-glucosidase to identify the potent inhibitor. An evaluation criterion was based on the binding affinities by using AutoDock. Results: The binding energy of Ellagic acid - Streptococcus mutans docked complex-10.63 kcal/mol and with Ellagic acid – Lactobacillus acidophilus docked complex was -7.30 kcal/mol. Conclusion: In this study, Showed that lesser binding energy better is the binding of the ligand and protein. These findings can provide a new strategy for dental caries disease therapy by using Ellagic acid as a inhibitor against  Streptococcus mutans and Lactobacillus acidophilus