Pregnancy-Associated Glycoprotein (PAG) merupakan molekul glikoprotein yang berkaitan<br />dengan kebuntingan. Molekul PAG disekresikan sebagai salah satu sinyal kebuntingan dari embrio kepada induk sebelum proses implantasi. Penelitian ini bertujuan untuk mengidentifikasi bobot molekul PAG pada kambing Jawarandu. Identifikasi PAG diperoleh dari ekstrak kotiledon yang dikumpukan setelah induk melahirkan. Proses penelitian dilakukan dengan empat tahap, yaitu ekstraksi, purifikasi larutan menggunakan presipitasi asam (H3PO4 1 M, pH 4.7) dan garam ((NH4)2SO4 40% dan 80%), filtrasi larutan menggunakan Sephadex G-75®, dan identifikasi bobot molekul PAG menggunakan SDS-PAGE. Bobot molekul protein yang muncul pada SDS-PAGE pada kolom K-8 menunjukkan adanya dua pita protein dengan bobot molekul 43,61 kDa dan 28,21 kDa. Molekul tersebut diduga dapat digunakan sebagai penanda kebuntingan dini pada kambing Jawarandu.<br />Kata kunci: PAG, Kotiledon, Kambing Jawarandu, SDS-PAGE
Pregnancy-Associated Glycoprotein (PAG) is a specific glycoprotein which associated to pregnancy. It is secreted by embryo as a signal of pregnancy before implantation. The aim of the study was to identify the molecular weight of PAG in Jawarandu goat. Placentas were obtained after labor to made the cotyledon extract. The study conducted through four stages, namely the extraction, purification performed using acid precipitation (H 3 PO 4 1 M, pH 4.7) and salt precipitation ((NH 4 ) 2 SO 4 40% dan 80%), filtration using Sephadex G-75 ® , and identification the molecular weight of PAG using SDS-PAGE. Protein bands appeared on SDS-PAGE in the K-8 column showed two protein bands with molecular weight namely 43.61 kDa and 28.21 kDa. These two molecule could be used as a marker of early pregnancy in Jawarandu goat.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2025 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.