This study was undertaken to assess the phytochemicals, vitamin A and vitamin C properties of different samples of smoothie produced from mixed tropical fruits flavoured with mint leaves extract. The smoothie was produced from three different fruits, (watermelon, apple and banana) then flavoured with mint leaf extract. The concentration of mint extract differs in each smoothie samples, ranging from 0-8% concentrate. The sample devoid of mint leaf extract served as control. The nine smoothie samples were examined for their phytochemical properties, vitamin A and vitamin C content. The study revealed that, the phytochemical content of different samples of smoothie gave these ranges: alkaloids (0.62-1.34%), saponin (1.07-1.46%), tannin (1.87-2.41%), phenol (0.02-0.41 mg/ml), flavonoid (1.62-3.33%), cyanogenic glycosides (0.00-0.05%). From the result, it was discovered that the phytochemical properties of the smoothie increases with increased mint leaf concentrate. The vitamins content of the samples ranges from; vitamin A (466-642 IU), vitamin C (9.30-10.83 mg/100 ml). The smoothie sample with high contents of mint extract are potentially good source of phytochemicals and vitamins.
Alcaligenes faecalis is a food borne transient microbe which has been reported to possess some antifungal properties. A. faecalis was assessed in vivo for use as a vaginal antifungal agent for the control of vaginal candidiasis. Fifty, three weeks old mice were used for the in vivo tests. Groups A and B were healthy and infected without treatment groups respectively. A 20 µl aliquot of 12.8 x 10 6 cfu/ml of C. albicans was used to elicit vaginal infections. Suppository clotrimazole was used as antifungal agent to control the infection in Group C mice, while a 20 µl aliquot of 10.8 x 10 8 cfu/ml of A. faecalis was infused into Groups D and E test mice and were used for treatment and prophylaxis of the infection respectively. Candidal counts, immunomodulatory roles of A. faecalis and vaginal histology were monitored through the experimental periods. There was a significant decrease (p≤0.05) in the candidal counts for groups C, D and E test mice. There was also significant increase (p≤0.05) in the white blood cell, lymphocyte and neutrophil counts of these same mice groups. Histopathology examination showed that A. faecalis could restore and also maintain normal vaginal architecture in Groups D and E respectively. These research findings show that A. faecalis possesses antifungal and immunomodulatory potentials for the control of vaginal cadidiasis infection.
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