A report on the thiamine, riboflavin, and niacin content of 3 varieties of California-grown walnuts of 4 successive crops was published in 1949 (8). Further studies in this laboratory have now been completed which have included analyses for pantothenic acid, folic acid, and vitamin B, as well as repetitions of the analyses for the three B vitamins previously studied.The new samples of Juglans regia were grown in the same districts and were shipped to the laboratory in the shells in the same manner previously described." The 3 varieties, Payne, Placentia, and Franquette, were the same used in the first study and samples of the 1950 and 1951 crops were examined. METHODSThe nuts were shelled, ground thoroughly twice, and divided into representatire samples which were packaged in pliofilm and stored in the freezer. All samples were made up of mixed light, amber, and dark kernels.The proximate analysis and vitamin determinations were done by the methods described by Hall, Morgan, and Wheeler ( 4 ) . The f a t content was expressed as total petroleum ether soluble content of the dry pulrerized samples.The thiochrome procedure, a combination of the Hennessey and Cerecedo ( 5 ) and Conner and Straub (3) methods, was used for thiamine with omission of the heating before the enzyme digestion with clarase. This produced a more rapid filtering, clearer filtrate than was found when the heating was done. The usual microbiological proeedures were used for riboflavin and niacin. There was, howerer, a change in the
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