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The aim of our research was to diminish the damaging effect of vaccination on the quality of stallion sperm and its cryostability. The task was to study the effect of the complex vitamin of tetrahydrovit on sperm parameters under conditions of vaccination against leptospirosis. Stallions that were vaccinated against leptospirosis were divided into 3 groups: the first –control, without vitamin supplements, the second – with the addition of 2 ml of tetrahydrovit vitamin daily, the third group – intramuscular injection of tetrahydrovit 1 time in 7 days. The reaction of the body to the introduction of the vaccine in the group without vitamins proceeded with a significant decrease in the quality of sperm in the first two weeks. Later, the motility and the number of whole membranes of fresh sperm gradually improved, but the survival rate of chilled and frozen sperm remained low until the end of the observations. Among stallions in the experimental groups, which were given tetrahydrovit vitamin (orally or intramuscularly), the decrease in sperm parameters was insignificant, within the usual fluctuations, which allowed one to avoid culling spermodoses. Tetrahydrovit vitamin promotes a stabilizing effect on the quality indicators of sperm of stallions-producers after vaccination.

The aim of the work is to find out the influence of vaccination of stallions against Siberian plague on the sperm quality and its criostability. Vaccination of stallions against Siberian plague led to the decrease of the sperm quality.During the first week productivity of sperm decreased in 10%. In freshly diluted sperm mobility decreased on average 12%, survival on 38% (Р0,99), membrane safety - on 20 % (Р0,95). Recovery was observed not earlier than in 28 days. Particularly sharply vaccination affected the sperm quality after crioconservation: sperm mobility decreased on average 35 % (Р0,99), survival on 45% (Р0,99), membrane safety on 46 % (Р0,99). Rejection of sperm doses in post-vaccination period were 50% because of the decrease of quality below normative level. Number of pathological types of sperm increased in 45 days after vaccination: on 27% (Р0,95) in fresh sperm and in 34% (Р0,99) in deconserved. In case of cryoconservation of stallions sperm it is necessary to take into account the recovery period of antiepizootic measures.