Effective method of obtaining of the bactericidal bandage materials by impregnation of cotton fabric by aqueous solutions of silver and copper salts followed by a certain regime of heat treatment is developed. The study of obtained materials by methods of optical spectroscopy, electron microscopy, and X-ray phase analysis showed the formation of crystalline silver nanoparticles (NPs) and bimetallic Ag/Cu composites with the corresponding surface plasmon resonance (SPR) bands in the absorption spectra. High antimicrobial and antimycotic properties of tissues with low concentrations of Ag and Ag/Cu nanoparticles (Ag/Cu NPs) (in the range 0.06–0.25 weight percent (wt%) for Ag and 0.015–0.13 wt% for Ag/Cu) is confirmed in experiments with a wide range of multidrug-resistant bacteria and fungi: Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, Klebsiella pneumoniae, Candida albicans yeasts, and micromycetes. Textile materials with Ag NPs demonstrate high antibacterial activity, while fabrics doped with bimetallic composite Ag/Cu have pronounced antimycotic properties. Bactericidal and antifungal properties of the obtained materials do not change after a washing. Production of such materials is extremely fast, convenient, and cost-effective.

No abstract

IFN-gamma and IFN-alpha production in vitro by peripheral blood cells activated by phytohemagglutinin or the Newcastle disease virus was impaired in patients with a benign process, cervical intraepithelial neoplasm and cancer in situ associated with human papillomavirus infection. In case of IFN-gamma and IFN-alpha production impairment following cervical papillomavirus infection, the increased severity of disease was accompanied by remarkable IFN system suppression. The lower synthesis of both IFN correlated with changes of some lymphocyte-subpopulation phenotype in peripheral blood. Lower CD4+ and CD3+ DR+ T cell concentrations were observed in papillomavirus-infected patients with impaired IFN production; impaired IFN-gamma production was accompanied by lower CD4/CD8 index.

Background and objective. Knowledge of local antimicrobial resistance pattern is very important for evidence-based empirical antibiotic prescribing. The main objective of the present study was to evaluate the prevalence and the antimicrobial resistance pattern of the main bacteria responsible for uncomplicated urinary tract infection (UTI) in Kyiv region (Ukraine), throughout a ten year period, in order to establish an appropriate empirical therapy. Materials and methods. A retrospective analysis of the etiological spectrum and antimicrobial resistance of uropathogens in urine samples isolated over the 10-year period, 2005 to 2015, in a single center was performed. Results. In total 380positive urine samples processed at our laboratory of which 193/380 (51 %) had E. coli as the infecting organism. Although E. coli was, as usual, the most common pathogen implicated in UTI, it were observed increasing the share of Enterococcus spp. - 82/380 (21.6%). Ampicillin and trimethoprim were the least-active agents against E. coli with resistance rates of 75% and 70%, respectively. Significant trends of increasing resistance over the 10-year period were identified for trimethoprim, fluoroquinolones ІІ and III generations, penicillins, and carbapenems. Nitrofuran derivative remains a reasonable empirical antibiotic choice in this community with a 10-year resistance rate of 8.3 %. Was determined that recurrent UTI is an independent risk factor for bacterial multidrug-resistance. Conclusions. Over the last 10 years, the proportions of fluoroquinolones resistant E. coli and multidrug-resistant bacteria have significantly increased. The fluoroquinolones shall not be used in the empirical treatment of uncomplicated UTI in Kyiv region patients. For the empirical treatment of uncomplicated UTI in women should be used nitrofuran derivative (furazydyn K). If required of parenteral administration of antibiotics should be used cephalosporins IVgeneration. This data will enable evidence-based empirical prescribing which will ensure more effective treatment and lessen the emergence of resistant uropathogens in the community.

No abstract