Background and Purpose-Aneurysm occlusion by intraluminal thrombus formation is the desired effect of all endovascular treatments. Intraluminal thrombus may, however, recanalize and be absorbed, unless it is infiltrated by cells that turn it into fibrous tissue (neointima). Because ruptured aneurysm walls are characterized by loss of smooth muscle cells, we assessed the impact of mural cell loss on wall remodeling of thrombosed aneurysms and investigated whether neointima formation could be enhanced by direct transplantation of cells into the thrombus. Methods-Sidewall aneurysms were microsurgically created in rats (n=81). Certain aneurysms were decellularized.Thrombosis was induced using direct injection of a fibrin polymer into the aneurysm. CM-Dil-labeled smooth muscle cells were injected into 25 of 46 fibrin embolized aneurysms. Recanalization and aneurysm growth were monitored with magnetic resonance angiography. Endoscopy, optical projection tomography, histology, and immunohistochemistry were used to study the fate of transplanted cells, thrombus organization, and neointima formation.

Aim. The clinical assessment of safety and hemostatic effect of «Haemoblock» hemostatic solution for arresting active bleeding from abdominal cavity tissues at open and laparoscopic surgeries.Methods. A total of 26 patients underwent surgeries. In 11 cases of acute calculous cholecystitis the drug was delivered to the bleeding surface during laparoscopic surgery by gauze soaked in «haemoblock» solution, which was introduced through 5 mm laparoscopic port (the first group). In 15 cases of acute adhesive intestinal obstruction, the drug was applied to the wound surface by moistened gauze during the open surgery (the second group).Results. In 23 (88.5%) cases of 26, stable hemostatic effect was obtained. Mean hemostasis time was 2.09±0.2 minutes in the first group and 2.53±0,15 minutes in the second group. The first group of patients, hemostasis occurred within 1-3 minutes in 10 (90.9%) of 11 patients, no secondary bleeding and suppuration was noticed. One patient (9.1%) of the first group developed recurrent bleeding from the gallbladder bed, requiring repeated surgery. Two (13.3%) patients of the second group had recurrent bleeding, requiring additional re-use of the drug with an exposure of 4 min. After using «Haemoblock», no postoperative complications such as fistula formation of adhesions, as well as reactive abdominal cavity effusions, were observed. No toxic of allergic reactions, irritating effect on the surrounding tissues no influence on systemic hemostasis were reported.Conclusion. «Haemoblock» is an effective local hemostatic that could be used as additional treatment to control parenchymal bleeding at abdominal and laparoscopic surgery.

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