2 Naučni institut za reprodukciju i v.o. domaćih životinja,Temerin, Srbija Izvod Ukupno je ispitano 14 različitih vrsta čajeva, i to: hajdučka trava, bosiljak, kantarion, pitoma nana, rastavić, kopriva, majkina dušica, kukuruzna svila, hibiskus, beli slez, kamilica, šipak-divlja ruža, vrijesak i divlja nana. Uzorci za merenje pripremljeni su metodom mikrotalasne digestije, a merenje je izvršeno sledećim metodama: masenom spektrometrijom sa indukovano spregnutom plazmom (Cd, Hg, Pb, As i Sb); atomskom apsorpcionom spektrometrijom (Mg); emisionom plamenom fotometrijom (Ca, K i Na) i spektrofotometrijom (P). Intervali varijacija (mg/kg) za makroelemente bili su: Ca (2738-35399); P (1545-6264); Mg (1647-7816); Na (293-10629) i K (9683-33985), a za toksične elemente: Cd (0,014-0,645); Hg (<0,001-0,017); Pb (0,064-1,340); As (0,030-0,544) i Sb (0,004-0,068). Treba istaći tri uzorka: hajdučka trava i dva uzorka kantariona u kojima je izmerena vrednost Cd (0,499± ±0,001; 0,539±0,002 i 0,645±0,003 mg/kg, redom) veća od 0,3 mg/kg, a koja predstavlja preporučeni dozvoljeni gornji nivo Cd u osušenom biljnom materijalu. Primenjena je Ward metoda hijerarhijske klaster analize, u cilju grupisanja biljnih čajeva po količini toksičnih elemenata.Ključne reči: biljni čajevi, makroelementi, toksični elementi, ICP-MS, AAS.

The health safety of honey includes the correctness in terms of the presence of different contaminants, which also implies the antibiotic residue. The presence of antibiotics in honey is prohibited, and methods of food analysis are prescribed in order to reliably determine antibiotics in food. In this paper the application of ELISA tests for determination of selected antibiotics and sulfonamides in honey is shown. The possibility of using four ELISA tests for the analysis of tetracyclines, streptomycines, chloramphenicol and sulfonamides was examined. Each test was evaluated after application on honey samples spiked with standard solution of a particular analyte. Samples were prepared according to the instructions of the ELISA test manufacturer referring to honey. Results of investigation of all ELISA tests, except for sulphonamides, have shown satisfactory accuracy (73-111%) and precision (14-16%). Recovery for sulfametoxypyridazin was low (40%), and for low tetracycline concentration was somewhat higher than acceptable (139%). The detection limits were in accordance with the limits given by the ELISA kit manufacturer and are also satisfactory in relation to the requirements of the legislation (0.075-3 µg/kg). The test kits can be used to screen the presence of tetracycline, chloramphenicol and streptomycin in the honey, taking into account the obtained validation parameters.

The content of selenium in basic broiler diets was 50 ± 10, 100 ± 10, 150 ± 10 and 250 ± 10 μgkg-1. At day 14, 28 and 42 tissue samples were collected. Dynamics and selenium deposits in blood, liver, muscle and heart were estimated in relation to the level of dietary selenium and age of broilers. When broilers were fed with diet containing 250 μgkg-1 Se, at day 42 the highest concentration of Se was in liver (570.8 ± 44.1 μgkg-1), blood (430.3 ± 46.5 μgL-1) and heart (237.5 ± 42.8 μgkg-1). At day 14, increase of dietary selenium content from 50 μgkg-1 to 250 μgkg-1 was followed by decrease of selenium deposits in heart: when broilers were fed with basic diet containing 50 μgkg-1 Se measured content of Se in heart muscle was 49.8 ± 15.6 μgkg-1 (99.6%) while in broilers fed with basic diet containing 250 μgkg-1 Se measured content of Se in heart was 147.2 ± 33.4 μgkg-1 (58.9%), respectively. The point of saturation as well as maximal concentration of selenium in liver was reached in period between fourth (556.7 ± 40.6 μgkg-1) and sixth (570.8 ± 44.1 μgkg-1) week of age. Also, feeding with basic diet containing 250 μgkg-1 Se the selenium blood level reached 162.8 ± 28.9 μgL-1 already at day 14 that represent 65.1% of dietary selenium

Mycotoxins, as secondary metabolic products of molds, are common contaminants of raw feed materials and compound feeds. Depending on the agro-meteorological and storage conditions, molds can contaminate grains and produce mycotoxins in the field, before and after harvest and during storage. Way of preventing animal mycotoxicoses and transfer of mycotoxins to humans through food chain is regular inspection on mycotoxicological feed safety. This paper presents the results of examination of aflatoxin, ochratoxin and zearalenone in 89 samples tested under laboratory conditions during a one year period. The analyses of types of samples, contamination, and their origin demonstrated the need for preventive control of mycotoxin content, primarily in grains. The results of testing the content of aflatoxin in milk indicate the existence of risks to human health. In order to protect humans and animals from mycotoxicological contamination, we propose the use of the system hazard analysis and critical control point (HACCP), which has been proved to be an effective strategy in food safety control. The basic principles of HACCP approach in the production of feed and foodstuffs, as well as the compliance and harmonization of legislations with those in the European Union, in the field of mycotoxicological tests, are the best prevention against mycotoxin effects on health and economy. Implementation of HACCP system currently presents one of the basic preconditions for the export of our products and their placement on the European market

Depending on the age and production category, cattle show different sensitivity towards certain mycotoxins. Microflora of the rumen degrades to a different degree and inactivates mycotoxins. In the work are presented the most important mycotoxicoses of cattle caused by fungal metabolites from the genera Fusarium, Aspergillus and Penicillium. Poisoning of cattle in our area is most often caused by Zearalenone, Dioxinivalenol, T-2 toxin, Ochratoxin A and Aflatoxin, but in the work are also presented Fumonisin B1 and B2. The work also describes preventive possibilities and protection of animal health from the effects of mycotoxins