Background: In rheumatoid arthritis, your immune system attacks the tissue lining the joints on both sides of your body. Other parts of the body may also be affected. Unsure of the exact cause. Two separate genes termed IL12A (p35) and IL12 encode the heterodimeric cytokine known as IL12 (p40). Several different hematopoietic cell types can have several different hematopoietic cell types that can generate antigen-presenting cells (APCs), including DCs and macrophages.Objectives: This study aimed to investigate if the interleukin IL-12B gene's common polymorphisms in an Iraqi population were associated with RA. Material and methods: Blood samples were taken from 70 Iraqi patients with RA illnesses and 30 Iraqi controls during the periods from April 2022 to June 2022 at Baghdad Teaching Hospital and Typical Rheumatology Unit. IL-12 level was determined by ELISA, and the IL-12B gene SNP was investigated through RT-PCR. Results: Between the sick and the healthy group, there was no statistically significant difference in the levels of IL-12. The allele G was more prevalent, and the genotype GG was more noticeable in patients compared to healthy people. As a result, the pattern represents a risk factor for RA (OR (95% CI, 1.55, (0.47 -5.12), P=0.523). Conclusion:We concluded that the IL-12B gene SNP rs3212227 GG was linked to the onset of RA, and that people carrying the G allele had a greater probability of doing so.

Background: Transition of medical students from a non-clinical to a clinical situation carries a considerable risk that needs further investigation. This study aims to detect and compare the throat bacterial colonization between medical students and patients' accompaniers in a tertiary hospital. Methods: Across-sectional descriptive study was conducted at the outpatient clinics of the Baquba Teaching Hospital at the Faculty of Medicine, Diyala University, Iraq.  A total of 120 throat swabs collected from a sample of 70 medical students (fifth stage) and 50 volunteers as a control group who were selected conveniently during their outpatient visits over September 2018. Aerobic and anaerobic culture methods were recruited to investigate the samples following the standard microbiological procedures. Results: Finding of this study indicate a high rate of bacterial throat colonization among medical students compared to the control group. Male gender showed high susceptibility for infection than females. The most common bacteria isolated among medical students were Staphylococcus aureus, and Escherichia coli 26 (37.1%), followed by Streptococcus pneumonia appeared in 23 samples (32.8%), Viridians streptococci 19 (27.1%), Acinetobacter spp.14 (20%), Enterobacter spp. 4 (5.7%), Candida spp. 3 (4.2%), Pseudomonas aeruginosa 2 (2.8%) respectively. Conclusion: Our findings suggest that medical students may contribute significantly to the transmission and dissemination of nosocomial pathogens among patients and vice versa.

Background: An autoimmune and inflammatory illness called rheumatoid arthritis (RA) occurs when your immune system mistakenly attacks normal cells in your body. Interleukin-35 is a brand-new cytokine that belongs to the immunosuppressive and anti-inflammatory IL-12 family. β -herpesvirus that produces inflammation and stays dormant in its host for life is the human cytomegalovirus. Human herpesvirus (HCMV) has been at the core of several RA-related theories. Objective: The current study looked at the association between RA and serum IL-35 levels as well as the association between RA and CMV. Patients and methods: Blood samples were taken in the Baghdad Teaching Hospital and Typical Rheumatology Unit from January 2022 to Mars 2022 for the current study, which comprised 70 Iraqi patients with RA illnesses and 22 Iraqi controls. ELISA was utilized to measure the biomarkers in this investigation, including the levels of IL-35 and anti-CMV lgG. Results: In comparison to control group, RA patients had increased levels of IL-35, but there was no statistical significant difference between the two groups. Because all participants in our research had anti-CMV IgG, it was determined that both sick and healthy individuals had previously been infected with the virus. Conclusion: Our results showed the role of IL-35 as a cytokine against inflammation and contributed to the control of RA pathogenesis, as well as the association between IL-35 and HCMV and whether the illness is active or not.

No abstract