Urine samples 96 to 100 were collected from patients (children) with urinary tract infections (UTIs) from Azadi Hospital in Kirkuk province, Iraq, where included (76 males and 120 females) with ages ranging from (≥ one-15) years old, for period from 1/1/2022 to 26/4/2022. The study included isolation and identifi cation depending on macroscopic, microscopic and defi nite with API 20e and API Staphylococcus. Moreover, all isolates were tested for resistance to 23 antibiotics. 55 bacterial isolations were obtained and E. coli had the highest rate of 27 (49.09%) followed by Klebsiella spp. with 14 (25.45%) and each of the bacteria, Staphylococcus aureus, Proteus spp., Citrobacter spp., Enterobacter spp., Pseudomonas spp. and Morganella morganii they reached 4(7.27%), 5(9.09%), 1(1.81%), 2 (3.63%), 1(1.81%) and 1(1.81%), respectively. Result showed enterobacteriaceae higher resistance to amoxicillin-clavulanate (100%). E. coli had the highest rate and were resistance 100% to tobramycin, penicillin and amoicillin, followed by amoxicillin-clavulanate and ampicillin (96.29 and 91.66), respectively. At the same time (Doxycycline, Ceftazidime, Tetracycline, Cefepime, Trimethoprim, Cefotaxime) were (88.88, 88, 85.71, 84, 83.33, 81.81%), respectively. At the same time, Pseudomonas spp. were resistant to (Ampicillin, Cefotaxime, Morganella morgani, and Erythromycin) 100% and S. aureus were resistant to (Amoxicillin-clavulanate, Cefotetan, Cefepime, Erythromycin, Norfl oxacin, Tetracycline, Cefotaxime, Morganella morgani, Amikacin) 100%.
