The present study was designed to determine the prevalence of sarcoptic mange from camels; evaluate the presence of secondary metabolites in the methanolic leaf extracts of D. angustifolia M. ferruginea, E. abyssinica, and E. globulus essential oil; and also evaluate their in vitro acaricidal efficacy against camel sarcoptic mange in the Babile District, Ethiopia. Each plant was subjected to qualitative phytochemical screening for the presence or absence of secondary metabolites. The in vitro application of each plant extracts was carried out against clinical mange mites with positive and negative controls. Accordingly, a total of 384 camels were examined for the presence of Sarcoptes scabiei var. cameli. The study revealed that an overall prevalence of 67(15.9%) animals was infested by mange mites. The study showed that a higher prevalence was observed in female camel than in male camel with no statistically significant difference ( P > 0.05 ). However, statistically higher prevalence ( P < 0.05 ) was recorded in young camel (28.2%) than adult (10.95%). The study also revealed that statistically higher prevalence ( P < 0.05 ) was observed in poor body conditioned camel (26.3%) than medium (13.5%) and good body condition camels (5.4%). For the in vitro evaluation of the efficacy of plant extracts, essential oils and crude extracts of four medicinal plant extracts were diluted at different concentrations from 200 mg/mL to 6.25 mg/mL and added to Petri dishes containing the adult stage of Sarcoptes scabiei. The efficacy of each plant was determined by comparing the mortality of mites treated with plant extracts with those of mites in nontreated (2% Tween 80) and treated (diazinon) control plates. After 3 h of contact, all concentrations of E. globulus, D. angustifolia, M. ferruginea, and E. abyssinica showed good in vitro acaricidal efficacy compared to controls nontreated control ( P < 0.05 ). After 3 h of exposure to the methanolic extract of D. angustifolia and the essential oil of E. globulus, comparable acaricidal efficacy in causing mites mortality at concentrations of 200 mg/mL has been recorded when compared to reference drugs. The crude extract of E. abyssinica showed a lower acaricidal efficacy compared to reference drugs. The present plant extracts studied showed comparable acaricidal efficacy to reference drugs at concentrations of 200 mg/mL. Therefore, further epidemiological and in vivo acaricidal studies as well as toxicity of the plants should be conducted in area to use these plants as alternative treatment option to substitute the use of synthetic drugs for which most of mites developed resistance and control impacts Sarcoptes scabiei on camel.

Background: Bovine mastitis is a major disease mostly caused by bacterial infection and associated with losses in the global dairy industry. Since mastitis-causing bacterial developing multidrug resistance to conventional antibiotics, there is an admirable supplementary study on medicinal plants to use them as an alternative therapy. This study aimed to evaluate the antibacterial activity and phytochemical screening of four selected medicinal plants against Staphylococcus aureus. Methods: An experimental study was done to evaluate the antibacterial activity of crude methanolic extracts of four traditionally used medicinal plants against S. aureus. Standard phytochemical screening tests were conducted to detect the bioactive principle of plants. Agar well diffusion assay was used to evaluate the antimicrobial activity of crude methanolic plant extract. The broth dilution method was also used to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of plant extracts. Results: The percentage yields obtained from the root of Clerodandrum myricoides, Kalanchoe densiflora, Kalanchoe marmorata and leaf of Kalanchoe marmorata and Datura stramonium were 22.6%, 37.2%, 51.6%, 32.3% and 50.7%, respectively. Phytochemical screening tests revealed the presence of secondary metabolites such as tannins, phenols, and steroids in all plant extract, except in D. stramonium, while others like alkaloids, flavonoids, quinones, and saponins were fairly detected in all samples. The agar well diffusion results showed significant (p < 0.05) differences in the mean zone of inhibition (ZOI) between each plant at different concentrations with significant potency comparable to gentamicin. C. myricoides and D. stramonium revealed the broadest spectrum of action yielding the highest ZOI (27.0 ± 0.58 mm), whereas K. marmorata leaf showed less activity with the lowest ZOI (22.3 ± 0.33 mm). The broth dilution method indicated that the MIC value of plant extracts against S. aureus ranged between 3.90 and 7.80 mg/ml while its corresponding MBC value ranged between 7.80 and 15.6 mg/ml. According to the MIC/MBC ratio, all tested plants (except K. densiflora) against standard S. aureus while C. myricoides and D. stramonium against clinical S. aureus isolate were determined to be bactericidal. Conclusion: This finding confirmed that all tested plants had a potential anti-staphylococcal effect. Thus, further study on in vivo experiments and cytotoxicity analyses must be conducted to suggest these plants as alternative mastitis treatments.

The current study was carried out to evaluate the anthelmintic efficacy of crude methanolic extracts of leaves of Croton macrostachyus and Nicotiana tabacum and rhizome parts of Zingiber officinale on nematode parasite, Haemonchus contortus. For these objectives, adult worm mortality assay (AWMA) was conducted on adult H. contortus to investigate the in vitro adulticidal effect of crude extracts from March to October 2021 G.C. The percentage yield of crude 99.9% methanolic extracts was 53%, 78%, and 44% for C. macrostachyus, N. tabacum, and Z. officinale, respectively. Phytochemical analysis using standard techniques was also used to detect secondary metabolites contained in the plants. The study revealed the presence of secondary metabolites such as tannins, flavonoids, steroids, and terpenoids in all extracts, which are considered to be the chemical components that are responsible for the wide therapeutic activities of several medicinal plants. In in vitro study, four graded concentrations of the crude extracts (500 mg/ml, 250 mg/ml, 125 mg/ml, and 62.5 mg/ml) were tested at regular time intervals, and parasite viability for 8 hours was recorded in triplicate. Albendazole (1.25 mg/ml) and distilled water were used as the positive and negative controls, respectively. At 4 hr posttreatment, the 62.5 mg/ml, 125 mg/ml, and 250 mg/ml concentrations of C. macrostachyus, N. tabacum, and Z. officinale extracts have caused significantly higher mortality ( P < 0.05 ) compared to the albendazole. Methanolic extracts of C. macrostachyus, N. tabacum, and Z. officinale produced mortality of adult H. contortus significantly ( P < 0.05 ) to the level of 93%, 83%, and 50% at concentration of 125 mg/ml at 4 hr posttreatment and meanwhile at 6 hr produced 100%, 100%, and 90%, respectively, at the same concentration. On the other hand, albendazole (1.25 mg/ml) killed 60% and 80% of the parasites at 4 hr and 6 hr posttreatment, respectively. Concentrations of all the extracts had showed a comparable and strong nematocidal effect on H. contortus having no significant difference with that of the positive control ( P > 0.05 ) at 8 hr posttreatment period. Hence, the current study revealed that the extracts from three plants have potential anthelmintic effect, and we recommend further study on fractionating each component separately and validating the materials using other parasite developmental stages are warranted.

A cross-sectional study was employed from March 2021 to October 2021 to isolate and identify Klebsiella species found in the gut of honey bees collected from worker of honey bee (Apis mellifera) from hives in Haramaya University bee farm, Damota and Finqile’s, managed under traditional and modern beekeeping apiculture. From the selected farm, a total of 60 samples of live adult honey bees were collected purposively. The live adult worker of the honey bee was individually surface-sterilized and complete alimentary canals of the worker bee were dissected and processed for Klebsiella isolation. Descriptive statistics were used to describe the occurrence of Klebsiella species and the proportion of Klebsiella found in the gut was analyzed for the association with study variables by the Pearson chi-square test. The overall prevalence of Klebsiella spp. was 50% from samples. The prevalence of Klebsiella pneumoniae was 26.7% and that of Klebsiella oxytoca was 23.3% from isolated using bacteriological examined samples. The isolates were characterized for the antimicrobial susceptibility test using the disc diffusion method. Among the isolated colonies, Klebsiella pneumoniae had the highest resistance to ampicillin (84.2%) and showed less resistance to gentamycin and trimethoprim sulfamethoxazole (26.3%). Klebsiella oxytoca was highly resistant to ampicillin (54.5%) and erythromycin (54.5%) and showed low and equal resistance to gentamycin and amoxicillin (18.2%). Molecular characterization should be conducted to identify Klebsiella spp. from honey bees. Monitoring antimicrobial effectiveness is recommended to tackle the existing problem in apiculture farms, and its possible public health threat should be noted for community by public health professionals.