No abstract

The attention has been paid in recent years to the biosynthesis of copper nanoparticles (CuNPs) and their important in medical applications. This study was conducted to synthesis of CuNPs using Aspergillus niger supernatant, study its characteristic and their antibacterial activity alone or with Ciprofloxacin against Staphylococcus aureus isolated from wounds infection. Then, its activity on the healing wounds was experimentally induced with Staphylococcus aureus using laboratory Hamster., followed by assaying of some blood parameters. The results indicated that Aspergillus niger supernatant was optimal for converting the CuNO3 to produce of CuNPs. The CuNPs were confirmed by UV spectrum, revealed an absorption at 225 nm, and the average size was by 20 nm after assay with the scanning electron microscopy (SEM). The mixing between CuNPs and ciprofloxacin had increased by 60% in the antibacterial activity against Staphylococcus aureus, more than for single inhibition activity of each treatment. The CuNPs or ciprofloxacin singly or in combination treated of induced experimentally contaminated wounds with Staphylococcus aureus appeared that synergism healing effects of the mixed treated with a short time compared with single treatments. The treatment of induced wounds with Staphylococcus aureus were significantly (p<0.05) increased in number of the WBCs and decreased of platelets number. While, the treatments with CuNPs or Ciprofloxacin singly or in combination were not significantly different with the same parameters in control group. Furthermore, the Hb, RBCs and PCV were not significantly different in all treatments. Furthermore, the values of urea and creatinine further of the IgG and IgA for all animals’ groups were reduced compared with the same values in infections animal group., GOT, GPT of liver enzymes values.were also showed a significant decreasing.

In the last decades, antibiotics were used to treat infections caused by some Staphylococcus species, especially Staphylococcus aureus and Staphylococcus epidermidis. The widespread use of antibiotics to treat staphylococcal infections has resulted in an increase in the resistance of bacteria to antibiotics, particularly to beta-lactam antibiotics. In recent years, researchers have been working on developing new antibiotics, despite the fact that they are complex and expensive and carry a number of risks associated with drug toxicity. Using new substances that have good potential against bacterial infection without causing bacteria to become resistant to these substances is currently being researched. More research has been carried out on the effect of silver and copper nanoparticles in neutralizing staphylococcal infection in laboratory studies. The toxic effect of nanoparticles was a concern to scientists, but despite that, the studies in vivo found that there was no toxic effect at low doses of nanoparticles on rats. The findings in this field were acceptable to entice researchers to develop these substances.