Thymus zygis subsp. gracilis, Mentha suaveolens and Sideritis incana (Lamiaceae family) are well recognized for their medicinal, pharmaceutical and aromatic properties. The present study aimed to investigate for the first time the phenolic composition, the antioxidant and antibacterial properties of the extracts obtained from the aerial parts of these species collected from the Ifrane region of Morocco. The phenolic compounds were determined using high‐performance liquid chromatography coupled to photodiode array and electrospray ionization mass spectrometry (HPLC‐PDA‐ESI/MS). The antioxidant activity was investigated using 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) radical scavenging, reducing power and ferrous ion chelating assays. The antibacterial activity was evaluated against three Gram‐negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhimurium) and three Gram‐positive bacteria (Staphylococcus aureus, Enterococcus faecalis and Listeria monocytogenes). A total of thirty‐three, sixteen and thirteen phenolic compounds were positively identified and characterized in T. zygis subsp. gracilis, M. suaveolens and S. incana extracts, respectively. Among the extracts, M. suaveolens exhibited the highest antioxidant activity, followed by S. incana and T. zygis subsp. gracilis in both DPPH and reducing power assays; all extracts showed the lowest activity in the chelating assay. In addition, all extracts demonstrated a bactericidal effect against Gram‐positive bacteria and bacteriostatic effect against Gram‐negative bacteria. Therefore, the aerial parts of Moroccan T. zygis subsp. gracilis, M. suaveolens and S. incana might be considered as a valuable source of natural antioxidant and antibacterial agents with potential application in food and pharmaceutical industries.

Objective: It's an ethnobotanical study to establish a floristic catalog of medicinal plants harvested from the province of Sidi Kacem (Morocco), which are used in traditional therapy for respiratory, digestive and skin infections Methods: We carried out a field survey using a questionnaire sent to 200 people spread over four studied areas, previously determined by stratified sampling techniques. This survey includes a section on the informant's therapeutic practices, and another on the varieties of plants used by the population Results:The analysis of the information found that 40% of the people surveyed practice herbal medicine. It enables us to establish a floristic catalog of 73 identified species. They belong to 39 families, the most dominant being Lamiaceae, Apiaceae and Asteraceae. The leaves are the most used part of the plant as a decoction Conclusion:These results are new; they allowed us for the first time to create a database on the floristic characteristics and the traditional therapy of this region. They will be useful for valorizing and rationalizing them by extending studies on their chemical properties and biological activities.