Partial dietary fish meal replacement with cotton seed meal and supplementation with exogenous protease alters growth, feed performance, haematological indices and associated gene expression markers (GH, IGF-I) for Nile tilapia, Oreochromis niloticus

No abstract

During the last 11 years, 1920 azoospermic men were examined and infertility was attributed to bilateral asplasia of vasa deferentia in 38 subjects (1.9% of cases). Epididymal biopsies showed sperm granulomas in the interductular spaces with increased phagocytosis. The sperm granuloma might be a factor in initiating humoral and cell mediated immune reaction, which interferes with sperm motility and fertilizing capacity of sperm. This would explain the poor pregnancy rate achieved with artificial insemination using sperm obtained from a created spermatocele in the head of the epididymis.

The aim of this study is to investigate the efficacy of WGO and Coenzyme Q10 low and high doses on physiological performance and testicular oxidative status of male rabbit bucks. In addition tracing the best suitable interval which modulates physiological response under Egyptian summer condition. This experiment was done on a pure strain of Sinai gabali bucks 3 months old. The animals were randomly divided into four groups each one comprise 6 animals. The 1 st group served as normal control (C), the 2 nd group treated with wheat germ oil (WGO) (300 mg/kg BW), the 3 rd group treated with Coenzyme Q10L (10 mg /kg BW), and the 4 th group treated with Coenzyme Q10H (20 mg/kg BW).Rabbits of all treatments were given oral administration daily for 60 days, and blood samples were collected monthly along 6 months after the end of the treatment. Moreover, the rabbits were decapitated and the testes tissues were exceed for evaluation Reduced Glutathione (GSH), Malondialdehyde (MDA), Oxidized Glutathione (GSSG) and Nitric oxide (NO) contents. Data revealed that liver function did not show any significant differences due to treatment. Month effect did not show any differences in ALT and AST values. According to the interaction of treatment and month data showed the best value of ALT and AST (26.48 and 32.72 U/L, respectively) in C× 6 th and Coenzyme Q10L ×5 th month, respectively. With regard to Lipid profile data showed significant decrease (P<0.05) of total cholesterol, LDL and triglyceride due to Coenzyme Q10L treatment and significant (P<0.05) increase in HDL due to Coenzyme Q10L treatment and the interaction showed the best values for TC, TG, HDL and LDL (89.50 mg/ dL Coenzyme Q10L×2 nd month, 87.50 mg/ dL Coenzyme Q10L×3 rd month, 36.00 mg/ dL Coenzyme Q10L×3 rd month and 35.96 mg/ dL Coenzyme Q10L×3 rd month, respectively). Results did not show any significant (P˃ 0.05) differences in blood proteins in the 4 groups due to the effect of treatments on male rabbits. Also month effect data did not show any changes (P˃ 0.05) in blood proteins. The interaction showed the best values of blood proteins, total protein ,albumin ,globulin and A/G ratio, 7.63g/dL in Coenzyme Q10H×4 th month (hot THI) , 4.17g/dL in WGO ×2 nd month (hot THI), 3.58g/dL in Coenzyme Q10H ×4 th month(hot THI) and 1.30 in Coenzyme Q10H ×6 th month (mild THI), respectively). Oxidative markers of testicular MDA and GSH showed decreases in MDA and increase in GSH due to WGO and Coenzyme Q10 treatments, respectively. In conclusion, the present study suggests that Coenzyme Q10 and WGO treatments improve physiological performance, reduced heat stress and testicular oxidative stress markers in Sinai gabali male rabbits under Egyptian summer condition.

The current study focused on evaluation of pomegranate peel extract (PPE) and the oxytetracycline as antioxidant and antiapoptotic potentialities on New Zealand White rabbits (NZW) performance, physiological homeostasis, hematobiochemical indices and stabilized DNA structure resembling in 8-hydroxy-2-deoxyguanosine (8OH2dG) level. For this purpose, four groups (each one compromise of 6 animals) of weaned NZW rabbits aged four weeks were randomly divided as follows: The 1 st group preserved as normal control (C) treated with tap water; 2 nd group (OXY) treated with daily oral dose of Oxytetracycline 200 mg/kg BW/day; 3 rd group (PPE) treated with daily oral dose of 130 mg/kg BW/day of PPE; 4 th group OXY + PPE treated with daily oral dose of 100 mg/kg BW/day of OXY + 65 mg/kg BW/day of PPE. The experiment lasted for 8 weeks. Concerning growth parameters, results showed that treatment with PPE and its combination with OXY enhanced body weight and ameliorate the negative effect on liver and kidney function with minimal side effects. In addition PPE elevate antioxidant parameters. However, OXY decreases the growth parameters (live body weight and body weight gain), total protein, albumin, globulin and A/G ratio levels and induces markedly increases in AST, ALT, creatinine, cortisol, and liver 8OH2dG concentrations. In conclusion, present study aimed to investigate the common side effects of oxytetracycline when compared with natural pomegranate peel extract and suggests that PPE and its combination with OXY at half dose of each ameliorate side effects of oxytetracycline, accelerate body weight and normalize physiological homeostasis.