Between 2014 and 2015, the European Centre for Disease Prevention and Control was informed of an increase in numbers of Salmonellaenterica serotype Chester cases with travel to Morocco occurring in six European countries. Epidemiological and microbiological investigations were conducted. In addition to gathering information on the characteristics of cases from the different countries in 2014, the epidemiological investigation comprised a matched case–case study involving French patients with salmonellosis who travelled to Morocco that year. A univariate conditional logistic regression was performed to quantify associations. The microbiological study included a whole genome sequencing (WGS) analysis of clinical and non-human isolates of S. Chester of varied place and year of isolation. A total of 162 cases, mostly from France, followed by Belgium, the Netherlands, Spain, Denmark and Sweden were reported, including 86 (53%) women. The median age per country ranged from 3 to 38 years. Cases of S. Chester were more likely to have eaten in a restaurant and visited the coast of Morocco. The results of WGS showed five multilocus sequence types (ST), with 96 of 153 isolates analysed clustering into a tight group that corresponded to a novel ST, ST1954. Of these 96 isolates, 46 (48%) were derived from food or patients returning from Morocco and carried two types of plasmids containing either qnrS1 or qnrB19 genes. This European-wide outbreak associated with travel to Morocco was likely a multi-source outbreak with several food vehicles contaminated by multidrug-resistant S. Chester strains.

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Many coastal cities around the world discharge their wastewaters into the marine environment. These wastewaters contain a high variety of pathogenic microorganisms that would have a role in the contamination of this ecosystem and may have potential risks for public health and environment. Using an environmental approach, we investigate the presence of Salmonella in wastewater treatment plants and its presence after the treatment in its receiving marine environment. In this environmental approach, we provide information about the inefficiency of wastewater treatment to remove Salmonella, especially that wastewater is considered as a good tank of high diversity of Salmonella serotypes. The identified Salmonella serotypes in the receiving marine environment almost coincide with those identified in wastewater. This characterization of Salmonella strains from wastewater and marine environment involves the direct impact of municipal wastewater discharges on this environment. Antibiotic susceptibility tests reveal generally the presence of multiresistant Salmonella strains in wastewater, which usually end up in the marine environment and may have a significant risk on the public health.

In order to use purified wastewater on watering green spaces of Agadir city, we conducted this study to assess the bacteriological and physicochemical quality of treated wastewater in Mzar treatment plant. To realize these objectives, we analyzed four types of waters: raw water (RW), decanted water, purified water and purified water treated by the ultraviolet radiation (PWUV). The biologically analyzed parameters are fecal coliforms (FC) and fecal enterococci (FE). The physicochemical parameters are: pH, electrical conductivity, chemical oxygen demand (COD), five-day biological oxygen demand (BOD 5) and total suspended solids (TSS). The results showed a high level of bacterial contamination in all types of waters, and the average loads of FC were between 4.71 × 10 6 CFU/100 mL (RW) and 1.45 × 10 3 CFU/100 mL (PWUV), while loads of FE were between 2.62 × 10 5 CFU/100 mL (RW) and 7.92 CFU/100 mL (PWUV). The physicochemical parameters show a pH close to neutrality and a high electrical conductivity (3261 μS/cm) in PWUV. In all stages of wastewater treatment, values of COD, BOD 5 and TSS were under the limit values recommended by Moroccan standards. Except for the electrical conductivity and FC, all the studied parameters were under the limit values requested by the Moroccan standards. The microbiological quality of PWUV is unstable, and if these waters are reused, they can affect the microbiological and physicochemical quality of green spaces and groundwater.

This research was carried out to assess the impact of treated wastewater irrigation on soil bacteriological and physicochemical properties and turfgrass bacteriological quality. Two golf courses were studied: a golf course A irrigated with freshwater (FW) and a golf course B irrigated with UV-treated wastewater (UV-TW). The physicochemical parameters (electrical conductivity and pH) of the soil were determined. FW, UV-TW, lake-stored water (LSW), turfgrass, and soil were collected, and their bacteriological parameters were determined. These parameters include: Escherichia coli, faecal enterococci, and faecal coliform. The results showed that the soil irrigated with treated wastewater (S-TW) showed a significant increase in the pH when compared with the soil irrigated with freshwater (S-FW). However, no significant difference was recorded in soil electrical conductivity. Faecal indicators concentration of the irrigation water samples varied considerably, and the concentrations in LSW frequently exceed those of the water at the output of the treatment plant (UV-TW). The comparison of the faecal contamination between the two golf courses indicates no significant difference in E. coli and faecal coliform concentrations. However, a significant difference was detected in faecal enterococci contamination. This study confirms that, under appropriate conditions, treated wastewater produced by M'zar wastewater treatment plant can be used as an alternative water resource for golf courses irrigation in Agadir city, Morocco.