Rumex nervosus is a genus of plant belongs to the family polygonaceae. In this study rumex nervosus represent as one of the selected plants used as a treatment for many diseases such as inflammatory and painful in Yemen. Extraction of this plant was by ethanolic and methanolic solvents. The obtained extract was tested for its antimicrobial activity against two Gram-positive, three Gram-negative and Candida albicans using agar diffusion method. However, S. aerus, E-Coli and Candida albicans showed the antimicrobial activity for ethanolic and methanolic solvents in (150mg).The results showed that this plant has antimicrobial activity against bacteria and fungi, so this plant is potential to be used as antimicrobial.