In Northern and Northeast Ghana, women accused of witchcraft are banished from society to live in special locations designated “witches camps.” The processes leading to their banishment, admission, and living in the camps may affect their psychological wellbeing and quality of life. This study was conducted to determine the prevalence of depression and assess the quality of life of 277 alleged witches in four camps located in these two regions in Ghana. A structured questionnaire was developed and administered using the open data collection kit (ODK). The Patient Health Questionnaire-8 (PHQ-8) and the World Health Organisation Quality of Life (WHOQOL) questionnaires were adopted to measure depression and health-related quality of life, respectively. The data were analysed using STATA version 16. The prevalence of depression among the alleged witches was 52.7%. Out of this, 37.2% had moderate depression, 7.2% had moderate or severe depression whilst 2.9% had severe depression. The sociodemographic factors that have a statistically significant association with depression included gender, marital status, being widowed or separated, and not having biological children. Over 97% of alleged witches have a low or extremely low quality of life. In conclusion, the majority of the people accused of witchcraft have a low or extremely low quality of life with high-probable depression.