The purpose of this research was to estimate the hepatoprotective efficacy of clay nanoparticles in improving the effects of anticancer drugs such as doxorubicin (DOX). One hundred male adult mice were randomly divided into five groups. Group 1 (control) was injected with double distilled water, Group 2 (EAC group) received intraperitoneal (IP) injection of 0.15 ml Ehrlich cells (2×10 6), in Group 3 (EAC+DOX group) EAC-bearing mice were treated with 0.07 ml of doxorubicin at a dose of 10 mg/kg bw. Doxorubicin was administered IP in six equal doses of injections to animals for 2 weeks for an accumulative dose of 10 mg/kg bw. Group 4 (EAC+DOX+MMT group) got i.p. infusion of 0.07 ml doxorubicin (10mg/kg) stacked on Montmorillonite nanoparticles (30 mg/kg) 3 times per week for about fourteen days. Group 5 (EAC+DOX+OCTA+MMT group) was gotten i.p. administration of 0.07 ml doxorubicin (10mg/kg) stacked on Octadecylamine (OCTA) with Montmorillonite nanoparticles (30 mg/kg) 3 times each week for about fourteen days. Blood samples were obtained at the end of the study and the serum was separated to measure liver function. The liver was removed and histologically analyzed. The serum levels of gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) were elevated, whereas the total protein and albumin were lower than control and other groups in the EAC. After handling of DOX clay NPs, the levels of these parameters were enhanced. In conclusion, nanoclays are influential in curing Ehrlich-induced ascites carcinoma in mice models in the Doxorubicin delivery system. They target DOX release in cancer cells and diminish the side effects of DOX in the liver.

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Background: Diazinon is the globally used organophosphorus compound to which humans are exposed through contamination of food and water. Flavonoids in Morus nigra exhibit a wide range of antioxidant, antimicrobial, anti-inflammatory, anti-cancer, anti-radiation properties and have a protective action against oxidative damage and hepatoprotective effects. The study's objective was to evaluate the effects of black mulberry (Morus nigra) extract against diazinon-induced hepatotoxicity in albino Wistar rats. Methodology: A quasi-experimental study was conducted from August 2019 to January 2020 at the Department of Pathology, Isra University, Hyderabad, Pakistan. Under standard colony conditions, thirty-six healthy male albino Wistar rats weighing between 200 and 300 grams were randomly divided equally into three groups: Group-1 (Control), Group-2 (given 60 mg/kg Diazinon daily for 4 weeks), Group-3 (administered Diazinon 60 mg/kg with 500 mg/kg of Morus nigra extract daily for 4 weeks through orogastric intubation). Hepatic tissue of all groups was observed under the light microscope. At the same time, hepatic serum markers were also analyzed. Results: A statistically significant decline in the bodyweight and rise in absolute liver weight of group-3 compared with groups-1 and group-2 (p<0.05). Co-administration of Morus nigra significantly lowered serum AST ad ALT levels. Significant histopathological derangement was observed in group-2 hepatic tissues while group-3 rats hepatic tissues had minimal changes and near-normal hepatic parenchyma. Conclusion: Morus nigra leaf extract has hepatoprotective effects against Diazinon-induced hepatotoxicity in male albino Wistar rats.