A series of sulfonamide derivatives HR1-HR5 were synthesized in one step reaction (nucleophilic substitution reaction SN 2 ). Structures of new products were confirmed by elemental and spectral analysis i.e., FTIR, UV, 1 H NMR, 13 C NMR, EIS-MS. In-vitro, antibacterial and anti-fungal activity of newly synthesized compounds was investigated against two bacterial strains: Escherichia coli and Staphylococcus aureus and two fungal strains: Aspergillum flavous and Aspergillum niger. It was found that among all tested compounds HR2 showed good antibacterial activity with MIC 1.13 × 10 -3 and 1.54 × 10 -3 for S. aureus and E. coli respectively. While HR4 showed good antifungal activity with inhibition zone 25.2 ± 0.12 mm (MIC: 71.2 × 10 -3 mol/L) and 17.1 ± 55.5 mm (MIC: 98.9 × 10 -3 mol/L) against A. flavous and A. niger respectively. Developed compounds were also screened for their Invitro antioxidant activity by DPPH radical scavenging assay. All compounds showed moderate activity but potential activity with 15.75% at 6 mM was exhibited by compound HR2.