Aim: To assess the knowledge, attitude and practice of healthcare professionals regarding the anaphylaxis in Medina, Saudi Arabia. Settings and Design: This was a population-based epidemiological study using a survey composed of 20 questions about the causes, symptoms, and treatment of anaphylaxis. Methods: This survey was distributed to healthcare professionals who represented different job categories including consultants, specialists, interns, residents, nurses, and medical students.  Statistical Analysis Used: One-way ANOVA is used to compare the knowledge score across different job categories. SPSS V26 software was used for the analysis and a p value of <0.05 was considered significant. Results: The mean of the correct answers for all participants was 60% and no difference in the mean scores was found in all participant from different job categories. The minimum achieved score was 30% and the maximum score was 100%. The score for the correct answers to each question was varied from 21% to 81%. Conclusions: The shortage of knowledge among the healthcare professionals regarding anaphylaxis predicts the occurrence of undesirable outcomes for patients. Educating healthcare professionals regarding the management of anaphylaxis can improve the quality of healthcare services.