Objective: This study aims to assess the knowledge and deficits of otolaryngology in primary care physicians who work at primary care centers or tertiary hospitals in Riyadh, KSA. Methods: This is a cross-sectional study conducted among residents of primary care specialties and otolaryngology residents in Riyadh, Saudi Arabia. A 10-item question with multiple-choice to assess the knowledge toward otolaryngology was given to the primary care provider by using face to face interview. Further, questions about clinical rotation in otolaryngology during medical school and residency were asked whether they participated or not. Results: A total of 253 primary care physicians took part (92 internal medicines, 79 family medicines, and 82 pediatrics) and 13 otolaryngologists, Primary care physicians who participated in clinical rotations during medical school and residency were 79.7% and 26.3%, respectively. Otolaryngologists' percentage of average correct knowledge score was (89.2%), family medicine (62.9%), pediatrics (55.4%), and internal medicine (52.5%). Primary care providers who participated in a clinical rotation in otolaryngology showed significantly higher knowledge scores than those who did not participate (p<0.001).Conclusion: primary care physicians had unsatisfactory ratings after examinations. Previous otolaryngology clinical rotation during residency had a positive effect on the knowledge of PCPs with their daily practice, which supports adding further clinical training in otolaryngology for primary care specialties.