A factorial experiment was conducted in alluvial mixture soil using the design (RCBD in research) Agricultural Research and Experiment Station - in the Al Bandar area located on the banks of the Euphrates River, affiliated to the College of Agriculture / University of Al-Muthanna for the winter agricultural season (2021-2022), with the aim of studying the effect of adding Trichoderma fungus harzianum and alga Chara sp and chemical fertilizers (0, 25, 50, 75) and the interaction between them in the concentration of NPK in the plant and the yield of the plant, and the results indicated the moral superiority of the double addition of the fungus T.harzianum and the alga Chara sp in all the studied traits of nitrogen concentration in the plant. (2.04%) the concentration of phosphorous in the plant (0.632), the concentration of potassium in the plant (2.235%), the number of grains of a spike (62.17 grains of a spike-1), and the weight of 1000 grains (52.36 g) of an enzyme, as significant differences were recorded. As a result of adding mineral fertilizer at different levels, level 75 recorded the highest values in nitrogen concentration in the plant (1.82%), phosphorous concentration in the plant (0.534%), potassium concentration in the plant (1.865%), the number of grains spike (56.94 grains of a spike-1) and weight 1000 tablets (51.39 g).