Background: The anterior cruciate ligament (ACL) is a band of dense connective tissue between the femur and the tibia. Many signs are associated with ACL injuries, which include joint effusion, altered movement, and decreased practical ability. This study aimed at assessing the knowledge and awareness of ACL injury among the Qassim region, Saudi Arabia, population. Methods: A cross-sectional study was carried out involving 383 participants, ranging in age from 18 to 65. Participants' sociodemographic characteristics were assessed with the help of a questionnaire with closed-ended questions. Data were analyzed using Statistical package for Social Sciences (SPSS) version 28. Results: Most respondents reported that ACL injuries to be a multi-factorial condition caused by multiple factors, including sports activity, lifting heavy weights, inadequate warming up, lack of awareness of protective methods, and being overweight. Furthermore, the results indicated that the respondent's attributed rest could decrease the pain associated with an ACL injury. Conclusion: The study revealed a significant association between age groups, gender, and sports participation. The study recommended that more knowledge and awareness be developed about ACL injury to help manage it and reduce its incidence.