Objective:The objective of this study was to evaluate the antibacterial activity of methanol extracts of oak, oak gall and whole fruitagainst number of Gram positive bacteria and Gram negative bacteria. Methods: The fresh fruits extracts (Oak, oak gall and whole fruit) were separately prepared with methanolby standard procedure. The samples were stored in refrigerator at 4°C until use. The bacterial samples were taken from Microbiology laboratory at the University of Raparin, Ranya-Kurdistan Region-Iraq. The antibacterial activity of different extracts of oak, oak gall and whole fruit was determined by using disc diffusion method. Results: The results of current study showed that all methanol extracts have effect on the growth of tested bacteria, except Gram negative bacteria. The study demonstrated that there was more significant effect on growth of gram positive bacteria (Staphylococcus sp, Streptococcus sp، Bacillus sp and Micrococcus sp)based on the inhibition zone diameter. Conclusion:This study indicates that allextracts mainly whole fruit have high antibacterial activity, and may serve as natural alternatives antibiotic against variety of bacterial infections caused by multi drug resistance Gram positive bacteria . More studies are require to isolate the active antibacterial agents in oak and oak gall so that they could use in pharmaceutical purposes.