Papaya (Carica Papaya L.) fruits index 2 were treated with 1.5, 2.5 and 3.5% solutions of calcium chloride by dipping and vacuum infiltration (-33 Kpa) or untreated (0%) as control. Effects of these treatments were evaluated on storage life and postharvest quality characteristics of papaya. After 21 days of storage at 13±1°C, the fruits were removed from storage for physicochemical analysis. Following additional five days holding in the storage condition for fruits used for evaluation of the rate of disease incidence and storage life. Postharvest dip treatments at different concentrations of calcium prolonged storage life, slowed down the ripening processes and maintained the quality of papaya. Whereas, it was effectively greater with calcium infiltration treatments than that for dip treatments. Calcium infiltration extended the storage life and retained the quality as calcium concentrations increased up to 2.5% and then declined. The desired effect was obtained at 2.5% infiltration compared with other treatments. The least disease incidence was found in those fruits infiltrated with 2.5% calcium. Hence, it can be concluded that postharvest infiltration of calcium at 2.5% has the potential to control disease incidence, prolong the storage life and preserve valuable attributes of postharvest papaya, presumably because of its effects on inhibition of ripening and senescence process and loss of the fruit firmness of papaya.
This is a review paper focusing on the acquisition of entrepreneurial skills in science through the authentic learning strategy to solve the youth unemployment problem in Nigeria. The youth unemployment in Nigeria is observed to be on the increase as indicated by statistics, and the various interventions seem not to be yielding significant results. It is on this background that the paper suggests the entrepreneurial skills through the authentic learning paradigm in Nigeria. The authors asserted that entrepreneurial skills are critical to job creation and self-employability because it enhances creativity and critical thinking skills. The authentic learning is a learning model that connects what the students learned in the classroom to the real-life, challenges and application. Some topics in science among many were identified in this thesis where students could acquire entrepreneurial skills that could make them selfemployed after graduation. The paper identified some implications of the paper and make suggestions for future studies.
Myrtaceae family is widely distributed in Asia has been the largest group of plant; mainly trees and few shrubs. Distributed all over the world in tropical and subtropical areas. Syzygium is the largest genus with economical value found all over the Malaysian Peninsular. Evolutionary relationships within the Syzygium is unclear and there are currently no reliable criteria to divide the genus into manageable entities for systematic study. Species of Syzygium is the richest genus of woody plants in South East Asia with approximately 1000 or more species but little is known about the genus. Syzygium polyanthum Wight is one of the favourites Ulam that have been consumed for ages in Peninsular Malaysia and also as herbal medicine. The species is widely misunderstood due to extreme morphological variability, similarity in aroma and flavor. The species is substituted or adulterated with several other species. The study was aimed to construct phenetic tree and unsupervised multivariate analysis from morphological and anatomical the data matrix. Phenetic analysis, Principal component and hierarchical cluster analysis revealed they are two different cultivars species but inter variation exited among cultivars of same species. The above documented information has added new taxonomic information with regard to the identification of the cultivars in Peninsular Malaysia. The study recommends further study on de novo sequence of Serai kayu and Serai kayu hutan.
Problem statement: Recurrent genital Chlamydia trachomatis infections due to treatment failures may results in complex sequelae leading to reproductive complexity and morbidity. It can be resulted by the heterotypic resistance with decreased drug susceptibility characteristic of the isolate. Studies are needed to understand the treatment failures and resistance characteristic of C. trachomatis. Hence, in vitro study was conducted on C. trachomatis isolate in the presence of antichlamydial drugs. Approach: Our aim was to study ygeD gene in C. trachomatis clinical isolate having decreased drug susceptibility profile and to analyze HeLa cells phenotypically upon infection in presence of antichlamydial drugs. Sequencing was done to check any mutational change (s) in ygeD gene of C. trachomatis isolate (CT-244), mRNA expression was analyzed in presence of antichlamydial drugs by Real Time RT-PCR. Transduction study was carried out in infected HeLa cells to detect changes at cellular level in presence of antichlamydial drugs by transducing with GFP/RFP-tagged proteins and analyzed by FACS. Results: A point mutation was detected in ygeD gene of C. trachomatis isolate. Further, mRNA expression level of ygeD gene was observed to be increased at 8 hpi in presence of doxycycline while in presence of azithromycin it was increased at 24 hpi. GFP-tagged plasma membrane protein expression in infected HeLa cells found to be reduced as compare to the uninfected cells. Upon infection, the RFP-tagged actin protein expression was up-regulated in comparison to the uninfected HeLa cells. No difference in expression of plasma membrane and actin protein was observed in susceptible serovar D and CT-244 isolate. Conclusion: The present study suggest that C. trachomatis isolate with decreased drug susceptibility profile may have an active efflux strategy for its survival in the presence of antichlamydial drugs and it may not affect its host cell plasma membrane or actin organization for its survival in order to resist the antichlamydial drugs
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